Comparative performance of Colisure

• Published in: Journal - American Water Works Association 1997-09, Vol.89 (9), p.112-120
• Main Authors: McFeters, Gordon A., Broadaway, Susan C., Pyle, Barry H., Pickett, Molly, Egozy, Yair
• Format: Article
• Language: English
• Subjects: Applied sciences; Bacteria; Bacteriological methods and techniques used in bacteriology; Bacteriological Techniques; Bacteriology; beta-Galactosidase - analysis; Biological and medical sciences; Biological and physicochemical phenomena; Bottles; Chlorination; Chlorine; Chlorine - adverse effects; Clinical Enzyme Tests; Coffee mugs; Coliform bacteria; Coliforms; Colony Count, Microbial; Comparability; Culture Media; Dehydration; Disinfectants - adverse effects; Drinking water; E coli; Effluents; Enterobacteriaceae - drug effects; Enterobacteriaceae - enzymology; Enterobacteriaceae - growth & development; Enterobacteriaceae - isolation & purification; Enzymes; Escherichia Coli; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - growth & development; Escherichia coli - isolation & purification; Exact sciences and technology; False Negative Reactions; Feces; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Lactose; Methods; Microbiology; Natural water pollution; Observational research; Pollution; Potable water; Sensitivity and Specificity; Sewage - microbiology; Sodium; Water Microbiology; Water Quality Monitoring; Water samples; Water Supply; Water treatment and pollution
• ISSN: 0003-150X; 1551-8833
• DOI: 10.1002/j.1551-8833.1997.tb08294.x

Colisure presence-absence medium was compared with standard reference methods for detecting low numbers of total coliform bacteria and E. coli in drinking water when the bacteria were subjected to chlorine stress. When Colisure was compared with established reference methods to detect total coliforms in dilute, disinfected samples, Colisure yielded more positive results after 24, 28, and 48 h than lauryl tryptose broth (LTB) confirmed in bile green lactose broth after 48 h. Colisure also detected higher levels of chlorine-injured E. coli than LTB confirmed in EC medium with 4-methylumbelliferyl B-D-glucuronide (EC/MUG). The sensitivity and specificity of Colisure were also evaluated and were determined to be between 96 and 100 percent on nonchlorinated samples when positive and negative tests were verified.