Luminescence Lifetime Imaging of Chemical SensorsA Comparison between Time-Domain and Frequency-Domain Based Camera Systems

Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compa...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-03, Vol.91 (5), p.3233-3238
Main Authors: Koren, Klaus, Moßhammer, Maria, Scholz, Vincent V, Borisov, Sergey M, Holst, Gerhard, Kühl, Michael
Format: Article
Language:English
Subjects:
Cameras
Chemical sensors
Chemistry
Frequency domain analysis
Imaging
Lifetime
Luminescence
Near infrared radiation
Organic chemistry
Time domain analysis
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Summary:Luminescence lifetime based imaging is still the most reliable method for generating chemical images using chemical sensor technology. However, only few commercial systems are available that enable imaging lifetimes within the relevant nanosecond to microsecond range. In this technical note we compare the performance of an older time-domain (TD) based camera system with a frequency-domain (FD) based camera system regarding their measuring characteristics and applicability for O2 and pH imaging in environmental samples and with different indicator dye systems emitting in the visible and near-infrared part of the spectrum. We conclude that the newly introduced FD imaging system delivers comparable if not better results than its predecessor, now enabling robust and simple chemical imaging based on FD luminescence lifetime measurements.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.8b05869