Reactivity of isolated human chorionic vessels: analysis of some influencing variables

• Published in: Canadian journal of physiology and pharmacology 2003-12, Vol.81 (12), p.1147-1151
• Main Authors: Abad, Antonio, Estañ, Luis, Morales-Olivas, Francisco J, Serra, Vicente
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Arteries - drug effects; Arteries - physiology; Biological and medical sciences; Cesarean Section; Chorion - blood supply; Delivery, Obstetric; Embryology: invertebrates and vertebrates. Teratology; Female; Fetal membranes; Fundamental and applied biological sciences. Psychology; General aspects. Development. Fetal membranes; Humans; In Vitro Techniques; Placenta - blood supply; Placentas; Potassium Chloride - pharmacology; Pregnancy; Pregnancy Trimester, Third; Reaction Time - drug effects; Reaction Time - physiology; Refrigeration; Vagina; Vein & artery diseases; Veins - drug effects; Veins - physiology
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/y03-119

The aim of the study was to determine whether 24 h of cold storage of samples, mode of delivery, and gestational age influenced in vitro human chorionic vascular reactivity (35 arteries and 34 veins). The following groups were compared: (i) fresh versus 24-h cold-stored (4 °C in Krebs-Henseleit solution) chorionic vascular rings from normal term placentas, (ii) fresh chorionic vascular rings from normal term placentas obtained after vaginal delivery versus those obtained after elective caesarean section, and (iii) fresh chorionic vascular rings from normal term placentas versus those obtained from preterm deliveries. Isometric recording of the concentration-response curve to KCl (5-120 mM) was assesed in each group. In vitro human chorionic vascular reactivity was influenced negatively by the 24-h cold storage of samples, with only 30% of stored samples being weakly reactive to KCl. Human chorionic vascular reactivity to KCl was unaffected by the mode of delivery. However, the response to KCl was gestational-age dependent. Thus, preterm vascular rings exhibited a significantly (P < 0.05) decreased response (E max = 9.8 ± 0.0 mN; EC50 = 26.0 ± 1.3 mM) compared with term samples (E max = 21.6 ± 2 mN; EC50 = 13.9 ± 1.6 mM). In conclusion, this study provides evidence that fresh term vascular rings are the tissues of choice for studying human chorionic vascular reactivity.Key words: human chorionic vessels, placenta, vascular reactivity.