Reduced activity of muscle Na+-K+-ATPase after prolonged running in rats

Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1 The purpose of this study was to investigate the hypothesis that Na + -K + -ATPase activity is reduced in muscle of different fiber composition after a single session of aerobic exercise in rats. In one experiment,...

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Published in:Journal of applied physiology (1985) 2002-11, Vol.93 (5), p.1703-1708
Main Authors: Fowles, J. R, Green, H. J, Schertzer, J. D, Tupling, A. R
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Membranes
Muscle Fibers, Fast-Twitch - enzymology
Muscle Fibers, Slow-Twitch - enzymology
Muscle, Skeletal - enzymology
Muscular system
Physical Endurance - physiology
Rats
Rats, Sprague-Dawley
Rodents
Running
Running - physiology
Sodium-Potassium-Exchanging ATPase - metabolism
Striated muscle. Tendons
Time Factors
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1 The purpose of this study was to investigate the hypothesis that Na + -K + -ATPase activity is reduced in muscle of different fiber composition after a single session of aerobic exercise in rats. In one experiment, untrained female Sprague-Dawley rats (weight 275 ± 21 g; means   ± SE; n  = 30) were run (Run) on a treadmill at 21 m/min and 8% grade until fatigue, or to a maximum of 2 h, which served as control (Con), or performed an additional 45 min of low-intensity exercise at 10 m/min (Run+). In a second experiment, utilizing rats of similar characteristics (weight 258 ± 18 g; n  = 32), Run was followed by passive recovery (Rec). Directly after exercise, rats were anesthetized, and tissue was extracted from Soleus (Sol), red vastus lateralis (RV), white vastus lateralis (WV), and extensor digitorum longus (EDL) and frozen for later analysis. 3- O -methylfluorescein phosphatase activity (3- O -MFPase) was determined as an indicator of Na + -K + -ATPase activity, and glycogen depletion identified recruitment of each muscle during exercise. 3- O -MFPase was decreased ( P  <   0.05) at Run+ by an average of 12% from Con in all muscles ( P  
ISSN:8750-7587
1522-1601
DOI:10.1152/japplphysiol.00708.2001