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Microbial degradation of four dispersed crude oils by Rhodococcus sp. evaluated using carbon stable isotope analysis
BACKGROUND The high frequency and wide distribution of oil spills pose serious threats to the marine environmental ecosystem and human health. Microbial degradation is considered to be the most applicable means to process oil spills. Carbon isotope fractionation was employed as a crucial tool for ev...
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| Published in: | Journal of chemical technology and biotechnology (1986) 2019-06, Vol.94 (6), p.1800-1807 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c3345-6e941916074f5116d8d1ba3915baca457d1e85de113edcd549d42da9ef899f173 |
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| cites | cdi_FETCH-LOGICAL-c3345-6e941916074f5116d8d1ba3915baca457d1e85de113edcd549d42da9ef899f173 |
| container_end_page | 1807 |
| container_issue | 6 |
| container_start_page | 1800 |
| container_title | Journal of chemical technology and biotechnology (1986) |
| container_volume | 94 |
| creator | Pi, Yongrui Chen, Bing Bao, Mutai Zhang, Baiyu |
| description | BACKGROUND
The high frequency and wide distribution of oil spills pose serious threats to the marine environmental ecosystem and human health. Microbial degradation is considered to be the most applicable means to process oil spills. Carbon isotope fractionation was employed as a crucial tool for evaluating the biodegradation of four crude oils by Rhodococcus sp. with Corexit 9500A as oil dispersant.
RESULTS
Following biodegradation, carbon isotope fractionation of n‐alkanes in four dispersed crude oil samples was measured by gas chromatography–isotope ratio mass spectrometry. A relationship between the carbon isotope ratios and residual concentrations of n‐alkanes was established based upon the Rayleigh equation, with a correlation coefficient, R2, ranging from 0.67 to 0.83. The carbon isotope enrichment factors for n‐alkanes of the four crude oil samples tended to be 0.04–0.25‰ in the residuals. The biodegradation level of n‐alkanes calculated from the Rayleigh equation agreed with the biodegradation results analyzed by gas chromatography–mass spectrometry.
CONCLUSIONS
The degree of microbial degradation was positively correlated to the carbon isotope fractionation in n‐alkanes. Compound‐specific stable isotope analysis is capable of quantifying the biodegradation level of crude oil or oil spills. © 2019 Society of Chemical Industry |
| doi_str_mv | 10.1002/jctb.5945 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2221740843</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2221740843</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3345-6e941916074f5116d8d1ba3915baca457d1e85de113edcd549d42da9ef899f173</originalsourceid><addsrcrecordid>eNp1kD1PwzAURS0EEqUw8A8sMTGk2I7txCNUfKoICZU5cuyX4irUwS8B9d-TUlamt5x3de8h5JyzGWdMXK1dX8-UkeqATDgzRSa1ZodkwoQuM6EKdUxOENeMMV0KPSH9c3Ap1sG21MMqWW_7EDc0NrSJQ6I-YAcJwVOXBg80hhZpvaWv79FHF50bkGI3o_Bl28H2Izdg2Kyos6keY7C3dQs0YOxjB9RubLvFgKfkqLEtwtnfnZK3u9vl_CFbvNw_zq8XmctzqTINRnLDNStkozjXvvS8trnhqrbOSlV4DqXywHkO3nkljZfCWwNNaUzDi3xKLva5XYqfA2BfrcdRYwmshBC8kKyU-Uhd7qlRBGKCpupS-LBpW3FW7aRWO6nVTurIXu3Z79DC9n-wepovb34_fgAyInr2</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2221740843</pqid></control><display><type>article</type><title>Microbial degradation of four dispersed crude oils by Rhodococcus sp. evaluated using carbon stable isotope analysis</title><source>Wiley</source><creator>Pi, Yongrui ; Chen, Bing ; Bao, Mutai ; Zhang, Baiyu</creator><creatorcontrib>Pi, Yongrui ; Chen, Bing ; Bao, Mutai ; Zhang, Baiyu</creatorcontrib><description>BACKGROUND
The high frequency and wide distribution of oil spills pose serious threats to the marine environmental ecosystem and human health. Microbial degradation is considered to be the most applicable means to process oil spills. Carbon isotope fractionation was employed as a crucial tool for evaluating the biodegradation of four crude oils by Rhodococcus sp. with Corexit 9500A as oil dispersant.
RESULTS
Following biodegradation, carbon isotope fractionation of n‐alkanes in four dispersed crude oil samples was measured by gas chromatography–isotope ratio mass spectrometry. A relationship between the carbon isotope ratios and residual concentrations of n‐alkanes was established based upon the Rayleigh equation, with a correlation coefficient, R2, ranging from 0.67 to 0.83. The carbon isotope enrichment factors for n‐alkanes of the four crude oil samples tended to be 0.04–0.25‰ in the residuals. The biodegradation level of n‐alkanes calculated from the Rayleigh equation agreed with the biodegradation results analyzed by gas chromatography–mass spectrometry.
CONCLUSIONS
The degree of microbial degradation was positively correlated to the carbon isotope fractionation in n‐alkanes. Compound‐specific stable isotope analysis is capable of quantifying the biodegradation level of crude oil or oil spills. © 2019 Society of Chemical Industry</description><identifier>ISSN: 0268-2575</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/jctb.5945</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Alkanes ; Biodegradation ; Carbon ; carbon isotope fractionation ; Carbon isotopes ; Chromatography ; Correlation coefficient ; Correlation coefficients ; Crude oil ; Dispersants ; dispersed crude oil ; Dispersion ; Environmental degradation ; Fractionation ; Gas chromatography ; Isotope fractionation ; Isotope ratios ; Isotopes ; Marine ecosystems ; Marine environment ; Mass spectrometry ; Mass spectroscopy ; Mathematical analysis ; Microbial degradation ; Microorganisms ; n‐alkanes ; Oil spills ; Organic chemistry ; Rayleigh equations ; Rhodococcus ; Scientific imaging ; Spectroscopy ; Stable isotopes</subject><ispartof>Journal of chemical technology and biotechnology (1986), 2019-06, Vol.94 (6), p.1800-1807</ispartof><rights>2019 Society of Chemical Industry</rights><rights>Copyright © 2019 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3345-6e941916074f5116d8d1ba3915baca457d1e85de113edcd549d42da9ef899f173</citedby><cites>FETCH-LOGICAL-c3345-6e941916074f5116d8d1ba3915baca457d1e85de113edcd549d42da9ef899f173</cites><orcidid>0000-0002-1595-4769 ; 0000-0001-8941-4258 ; 0000-0002-6997-4765</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Pi, Yongrui</creatorcontrib><creatorcontrib>Chen, Bing</creatorcontrib><creatorcontrib>Bao, Mutai</creatorcontrib><creatorcontrib>Zhang, Baiyu</creatorcontrib><title>Microbial degradation of four dispersed crude oils by Rhodococcus sp. evaluated using carbon stable isotope analysis</title><title>Journal of chemical technology and biotechnology (1986)</title><description>BACKGROUND
The high frequency and wide distribution of oil spills pose serious threats to the marine environmental ecosystem and human health. Microbial degradation is considered to be the most applicable means to process oil spills. Carbon isotope fractionation was employed as a crucial tool for evaluating the biodegradation of four crude oils by Rhodococcus sp. with Corexit 9500A as oil dispersant.
RESULTS
Following biodegradation, carbon isotope fractionation of n‐alkanes in four dispersed crude oil samples was measured by gas chromatography–isotope ratio mass spectrometry. A relationship between the carbon isotope ratios and residual concentrations of n‐alkanes was established based upon the Rayleigh equation, with a correlation coefficient, R2, ranging from 0.67 to 0.83. The carbon isotope enrichment factors for n‐alkanes of the four crude oil samples tended to be 0.04–0.25‰ in the residuals. The biodegradation level of n‐alkanes calculated from the Rayleigh equation agreed with the biodegradation results analyzed by gas chromatography–mass spectrometry.
CONCLUSIONS
The degree of microbial degradation was positively correlated to the carbon isotope fractionation in n‐alkanes. Compound‐specific stable isotope analysis is capable of quantifying the biodegradation level of crude oil or oil spills. © 2019 Society of Chemical Industry</description><subject>Alkanes</subject><subject>Biodegradation</subject><subject>Carbon</subject><subject>carbon isotope fractionation</subject><subject>Carbon isotopes</subject><subject>Chromatography</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Crude oil</subject><subject>Dispersants</subject><subject>dispersed crude oil</subject><subject>Dispersion</subject><subject>Environmental degradation</subject><subject>Fractionation</subject><subject>Gas chromatography</subject><subject>Isotope fractionation</subject><subject>Isotope ratios</subject><subject>Isotopes</subject><subject>Marine ecosystems</subject><subject>Marine environment</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Mathematical analysis</subject><subject>Microbial degradation</subject><subject>Microorganisms</subject><subject>n‐alkanes</subject><subject>Oil spills</subject><subject>Organic chemistry</subject><subject>Rayleigh equations</subject><subject>Rhodococcus</subject><subject>Scientific imaging</subject><subject>Spectroscopy</subject><subject>Stable isotopes</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kD1PwzAURS0EEqUw8A8sMTGk2I7txCNUfKoICZU5cuyX4irUwS8B9d-TUlamt5x3de8h5JyzGWdMXK1dX8-UkeqATDgzRSa1ZodkwoQuM6EKdUxOENeMMV0KPSH9c3Ap1sG21MMqWW_7EDc0NrSJQ6I-YAcJwVOXBg80hhZpvaWv79FHF50bkGI3o_Bl28H2Izdg2Kyos6keY7C3dQs0YOxjB9RubLvFgKfkqLEtwtnfnZK3u9vl_CFbvNw_zq8XmctzqTINRnLDNStkozjXvvS8trnhqrbOSlV4DqXywHkO3nkljZfCWwNNaUzDi3xKLva5XYqfA2BfrcdRYwmshBC8kKyU-Uhd7qlRBGKCpupS-LBpW3FW7aRWO6nVTurIXu3Z79DC9n-wepovb34_fgAyInr2</recordid><startdate>201906</startdate><enddate>201906</enddate><creator>Pi, Yongrui</creator><creator>Chen, Bing</creator><creator>Bao, Mutai</creator><creator>Zhang, Baiyu</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-1595-4769</orcidid><orcidid>https://orcid.org/0000-0001-8941-4258</orcidid><orcidid>https://orcid.org/0000-0002-6997-4765</orcidid></search><sort><creationdate>201906</creationdate><title>Microbial degradation of four dispersed crude oils by Rhodococcus sp. evaluated using carbon stable isotope analysis</title><author>Pi, Yongrui ; Chen, Bing ; Bao, Mutai ; Zhang, Baiyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3345-6e941916074f5116d8d1ba3915baca457d1e85de113edcd549d42da9ef899f173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alkanes</topic><topic>Biodegradation</topic><topic>Carbon</topic><topic>carbon isotope fractionation</topic><topic>Carbon isotopes</topic><topic>Chromatography</topic><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Crude oil</topic><topic>Dispersants</topic><topic>dispersed crude oil</topic><topic>Dispersion</topic><topic>Environmental degradation</topic><topic>Fractionation</topic><topic>Gas chromatography</topic><topic>Isotope fractionation</topic><topic>Isotope ratios</topic><topic>Isotopes</topic><topic>Marine ecosystems</topic><topic>Marine environment</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Mathematical analysis</topic><topic>Microbial degradation</topic><topic>Microorganisms</topic><topic>n‐alkanes</topic><topic>Oil spills</topic><topic>Organic chemistry</topic><topic>Rayleigh equations</topic><topic>Rhodococcus</topic><topic>Scientific imaging</topic><topic>Spectroscopy</topic><topic>Stable isotopes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pi, Yongrui</creatorcontrib><creatorcontrib>Chen, Bing</creatorcontrib><creatorcontrib>Bao, Mutai</creatorcontrib><creatorcontrib>Zhang, Baiyu</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pi, Yongrui</au><au>Chen, Bing</au><au>Bao, Mutai</au><au>Zhang, Baiyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbial degradation of four dispersed crude oils by Rhodococcus sp. evaluated using carbon stable isotope analysis</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><date>2019-06</date><risdate>2019</risdate><volume>94</volume><issue>6</issue><spage>1800</spage><epage>1807</epage><pages>1800-1807</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><abstract>BACKGROUND
The high frequency and wide distribution of oil spills pose serious threats to the marine environmental ecosystem and human health. Microbial degradation is considered to be the most applicable means to process oil spills. Carbon isotope fractionation was employed as a crucial tool for evaluating the biodegradation of four crude oils by Rhodococcus sp. with Corexit 9500A as oil dispersant.
RESULTS
Following biodegradation, carbon isotope fractionation of n‐alkanes in four dispersed crude oil samples was measured by gas chromatography–isotope ratio mass spectrometry. A relationship between the carbon isotope ratios and residual concentrations of n‐alkanes was established based upon the Rayleigh equation, with a correlation coefficient, R2, ranging from 0.67 to 0.83. The carbon isotope enrichment factors for n‐alkanes of the four crude oil samples tended to be 0.04–0.25‰ in the residuals. The biodegradation level of n‐alkanes calculated from the Rayleigh equation agreed with the biodegradation results analyzed by gas chromatography–mass spectrometry.
CONCLUSIONS
The degree of microbial degradation was positively correlated to the carbon isotope fractionation in n‐alkanes. Compound‐specific stable isotope analysis is capable of quantifying the biodegradation level of crude oil or oil spills. © 2019 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jctb.5945</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-1595-4769</orcidid><orcidid>https://orcid.org/0000-0001-8941-4258</orcidid><orcidid>https://orcid.org/0000-0002-6997-4765</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0268-2575 |
| ispartof | Journal of chemical technology and biotechnology (1986), 2019-06, Vol.94 (6), p.1800-1807 |
| issn | 0268-2575 1097-4660 |
| language | eng |
| recordid | cdi_proquest_journals_2221740843 |
| source | Wiley |
| subjects | Alkanes Biodegradation Carbon carbon isotope fractionation Carbon isotopes Chromatography Correlation coefficient Correlation coefficients Crude oil Dispersants dispersed crude oil Dispersion Environmental degradation Fractionation Gas chromatography Isotope fractionation Isotope ratios Isotopes Marine ecosystems Marine environment Mass spectrometry Mass spectroscopy Mathematical analysis Microbial degradation Microorganisms n‐alkanes Oil spills Organic chemistry Rayleigh equations Rhodococcus Scientific imaging Spectroscopy Stable isotopes |
| title | Microbial degradation of four dispersed crude oils by Rhodococcus sp. evaluated using carbon stable isotope analysis |
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