Bacillus cereus biofilm formation on central venous catheters of hospitalised cardiac patients

Formation of bacterial biofilms is a risk with many in situ medical devices. Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2019-02, Vol.35 (2), p.204-216
Main Authors: Ikram, Samman, Heikal, Adam, Finke, Sarah, Hofgaard, Antje, Rehman, Yasir, Sabri, Anjum Nasim, Økstad, Ole Andreas
Format: Article
Language:English
Subjects:
Ampicillin
Bacillus cereus
Bacillus cereus group
Bacteria
Biofilm
Biofilms
cardiac patients
Catheters
central venous catheters
Chromosomes
Clindamycin
comparative genomics
Contamination
DNA
Gene sequencing
Genes
Genomes
Heart
Immunocompromised hosts
Inspection
Medical devices
Medical electronics
Medical equipment
Medical instruments
Microbiological strains
Nucleotide sequence
Patients
PCR
Penicillin
Phylogeny
Risk analysis
Risk factors
Sulfonamides
Trimethoprim
whole genome sequencing
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Summary:Formation of bacterial biofilms is a risk with many in situ medical devices. Biofilm-forming Bacillus species are associated with potentially life-threatening catheter-related blood stream infections in immunocompromised patients. Here, bacteria were isolated from biofilm-like structures within the lumen of central venous catheters (CVCs) from two patients admitted to cardiac hospital wards. Isolates belonged to the Bacillus cereus group, exhibited strong biofilm formation propensity, and mapped phylogenetically close to the B. cereus emetic cluster. Together, whole genome sequencing and quantitative PCR confirmed that the isolates constituted the same strain and possessed a range of genes important for and up-regulated during biofilm formation. Antimicrobial susceptibility testing demonstrated resistance to trimethoprim-sulphamethoxazole, clindamycin, penicillin and ampicillin. Inspection of the genome revealed several chromosomal β-lactamase genes and a sulphonamide resistant variant of folP. This study clearly shows that B. cereus persisting in hospital ward environments may constitute a risk factor from repeated contamination of CVCs.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2019.1586889