Pyridoxalated hemoglobin polyoxyethylene conjugate reverses hyperdynamic circulation in septic sheep

• Published in: Journal of applied physiology (1985) 1998-06, Vol.84 (6), p.1991-1999
• Main Authors: Bone, Hans G, Schenarts, Paul J, Fischer, Stefanie R, McGuire, Roy, Traber, Lillian D, Traber, Daniel L
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Bacterial diseases; Biochemistry; Biological and medical sciences; Blood; Blood Cell Count; Blood pressure; Blood Pressure - drug effects; Blood Pressure - physiology; Blood Substitutes - pharmacology; Cardiac Output - drug effects; Cardiac Output - physiology; Circulatory system; Experimental bacterial diseases and models; Female; Heart Rate - drug effects; Heart Rate - physiology; Hemodynamics - drug effects; Hemodynamics - physiology; Hemoglobin; Hemoglobins - pharmacology; Infectious diseases; Kidneys; Liver Circulation - drug effects; Liver Circulation - physiology; Medical sciences; Pancreas - blood supply; Pancreas - drug effects; Polyethylene Glycols - pharmacology; Pseudomonas Infections - microbiology; Pseudomonas Infections - physiopathology; Regional Blood Flow - drug effects; Renal Circulation - drug effects; Renal Circulation - physiology; Sepsis - metabolism; Sepsis - microbiology; Sepsis - physiopathology; Sheep; Vascular Resistance - drug effects; Vascular Resistance - physiology; Vasoconstriction - drug effects
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/jappl.1998.84.6.1991

1  Department of Anesthesiology, Westfälische-Wilhelms-Universität, Münster, Germany 48149; and 2  Departments of Anesthesiology, Physiology, and Biophysics, The University of Texas Medical Branch, and The Shriners Burns Institute, Galveston, Texas 77555-0833 We investigated the effects of modified hemoglobin on regional blood flow and function of different organs during hyperdynamic sepsis. Fourteen sheep were surgically prepared for the study. After a 5-day recovery period, a continuous infusion of live Pseudomonas aeruginosa bacteria was begun and maintained for 48 h. At 24 h, after a hyperdynamic circulation had developed, the animals were randomly assigned to two groups: 1 ) a treatment group ( n  = 7) that received an infusion with 100 mg/kg pyridoxalated hemoglobin polyoxyethylene conjugate (PHP) over 30 min and 2 ) a control group ( n  = 7) that received only the vehicle. PHP infusion increased mean arterial pressure from 86 ± 2.8 to 101.8 ± 3.5 mmHg ( P  < 0.05) and systemic vascular resistance index from 769 ± 42.1 to 1,087 ± 56.8 dyn · s · m 2  · cm 5 ( P