A comparison of voluntary and electrically induced contractions by interleaved 1H- and 31P-NMRS in humans

• Published in: Journal of applied physiology (1985) 2003-03, Vol.94 (3), p.1012-1024
• Main Authors: Vanderthommen, M, Duteil, S, Wary, C, Raynaud, J. S, Leroy-Willig, A, Crielaard, J. M, Carlier, P. G
• Format: Article
• Language: English
• Subjects: Adenosine Diphosphate - metabolism; Adenosine Triphosphate - metabolism; Adult; Biological and medical sciences; Electric Stimulation; Energy Metabolism - physiology; Exercise; Exercise - physiology; Fundamental and applied biological sciences. Psychology; Glycolysis - physiology; Humans; Hydrogen-Ion Concentration; Ischemia - metabolism; Ischemia - physiopathology; Isometric Contraction - physiology; Magnetic Resonance Spectroscopy; Male; Muscle Contraction - physiology; Muscle Fibers, Skeletal - metabolism; Muscle Fibers, Skeletal - physiology; Muscle, Skeletal - chemistry; Muscle, Skeletal - cytology; Muscle, Skeletal - physiology; Muscular system; Myoglobin - metabolism; Oxygen Consumption - physiology; Phosphocreatine - metabolism; Recruitment, Neurophysiological - physiology; Spectrum analysis; Striated muscle. Tendons; Vertebrates: osteoarticular system, musculoskeletal system
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00887.2001

1  Physical Medicine Department, University Hospital, 4000 Liège, Belgium; and 2  NMR Laboratory, AFM and CEA, Institute of Myology, Pitié-Salpêtrière University Hospital, 75651 Paris, France Skeletal muscle voluntary contractions (VC) and electrical stimulations (ES) were compared in eight healthy men. High-energy phosphates and myoglobin oxygenation were simultaneously monitored in the quadriceps by interleaved 1 H- and 31 P-NMR spectroscopy. For the VC protocol, subjects performed five or six bouts of 5 min with a workload increment of 10% of maximal voluntary torque (MVT) at each step. The ES protocol consisted of a 13-min exercise with a load corresponding to 10% MVT. For both protocols, exercise consisted of 6-s isometric contractions and 6-s rest cycles. For an identical mechanical level (10% MVT), ES induced larger changes than VC in the P i -to-phosphocreatine ratio [1.38 ± 1.14 (ES) vs. 0.13 ± 0.04 (VC)], pH [6.69 ± 0.11   (ES) vs. 7.04 ± 0.07 (VC)] and myoglobin desaturation [43 ± 15.9   (ES) vs. 6.1 ± 4.6% (VC)]. ES activated the muscle facing the NMR coil to a greater extent than did VCs when evaluated under identical technical conditions. This metabolic pattern can be interpreted in terms of specific temporal and spatial muscle cell recruitment. Furthermore, at identical levels of energy charge, the muscle was more acidotic and cytoplasm appeared more oxygenated during ES than during VC. These results are in accordance with a preferential recruitment of type II fibers and a relative muscle hyperperfusion during ES. muscle metabolism; electrical stimulation; high-energy phosphates; myoglobin oxygenation; human; nuclear magnetic resonance spectroscopy