Proteome analysis of muscadine grape leaves

Muscadine grapes are native to the southeastern United States and are used for making wine and consumed as fresh fruit. Grape berries, as 'sink organs,' rely on the use of available carbohydrate resources produced by photosynthesis to support their development and composition. A high throu...

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Bibliographic Details
Published in:International journal of wine research 2009, Vol.1, p.161
Main Author: Sheikh, Mehboob B.
Format: Article
Language:English
Subjects:
Analysis
Biopolymers
Cultivars
Electrophoresis
Fruits (Food)
Genotypes
Glutamine
Grapes
Ligases
Photosynthesis
Physiological aspects
Plant biochemistry
Polypeptides
Proteins
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Summary:Muscadine grapes are native to the southeastern United States and are used for making wine and consumed as fresh fruit. Grape berries, as 'sink organs,' rely on the use of available carbohydrate resources produced by photosynthesis to support their development and composition. A high throughput two-dimensional gel electrophoresis (2-DE) was conducted on muscadine (Vitis rotundifolia) grape leaf proteins to document complexity in their composition and to determine protein identity and function for enhancing photo synthetic efficiency of muscadine grape. 2-DE resolved muscadine leaf proteins into >258 polypeptides with pIs between 3.5 and 8.0 and molecular weight between 12,000 to 15,0000 Daltons. The consistently expressed proteins were excised and subjected to sequencing. Homology search of protein sequences showed 84% identity with Viridi plantae database. Identity of some of these proteins included RuBisCO, glutamine synthetase, pathogenesis-related protein, glyoxisomal malate dehydrogenase, ribonucleoprotein, chloroplast precursor, oxygen evolving enhancer protein. Comparative analysis of 10 muscadine cultivars showed quantitative differences in expression of 39 polypeptides among these genotypes. The results suggested that the polypeptide composition of muscadine grape leaf is complex, and polypeptide number and amount vary widely among muscadine genotypes, and these variations may be responsible for differences in their physiology, berry and stress tolerance characteristics. Keywords: grapevine, leaves, muscadine, proteins, sequencing, 2-DE
ISSN:1179-1403
1179-1403
DOI:10.2147/IJWR.S5574