Mechanism Underlying the Effect of Mecamylamine on Nicotinic Acetylcholine Receptors of Rat Sympathetic Ganglion Neurons

On neurons of the superior cervical ganglion of 3-week-old rats, we studied the mechanism underlying the blocking effect of mecamylamine on transmembrane currents evoked by iontophoretic application of acetylcholine (ACh currents); these currents were recorded with the use of a patch-clamp technique...

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Bibliographic Details
Published in:Neurophysiology (New York) 2001-01, Vol.33 (1), p.23
Main Authors: Purnyn', E E, Voitenko, S V, Remizov, I N, Rikhal'sky, O V, Skok, V I
Format: Article
Language:English
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Summary:On neurons of the superior cervical ganglion of 3-week-old rats, we studied the mechanism underlying the blocking effect of mecamylamine on transmembrane currents evoked by iontophoretic application of acetylcholine (ACh currents); these currents were recorded with the use of a patch-clamp technique in the whole-cell configuration. The IC50 of the above agent equaled (2.7 \pm 0.3) [middot] 10-10 M. The blocking effect of mecamylamine on ACh current did not depend on the membrane potential and decreased with rise in the concentration of the drug. Thus, a competitive blocking mechanism mostly underlies the above phenomenon.
ISSN:0090-2977
1573-9007
DOI:10.1023/A:1010408113230