Hepatic and gastroprotective activity of Serjania marginata leaf aqueous extract in Nile tilapia (Oreochromis niloticus)

• Published in: Fish physiology and biochemistry 2019-06, Vol.45 (3), p.1051-1065
• Main Authors: do Carmo Ota, Erika, Honorato, Claucia Aparecida, Heredia-Vieira, Sílvia Cristina, Flores-Quintana, Carolina Isabel, de Castro Silva, Tarcila Souza, Inoue, Luis Antonio Kioshi Aoki, Cardoso, Claudia Andrea Lima
• Format: Article
• Language: English
• Subjects: Abnormalities; Alkaline phosphatase; Anatomical structures; Animal Anatomy; Animal Biochemistry; Animal Physiology; Aquaria; Aquariums; Biomedical and Life Sciences; Digestive enzymes; Enzymatic activity; Enzyme activity; Enzymes; Fasting; Feeds; Fibrosis; Fish; Freshwater & Marine Ecology; Freshwater fishes; Goblet cells; Hepatocytes; Histology; Histopathology; Identification; Intestine; Intestines; Leaves; Levels; Life Sciences; Lipase; Liver; Malformations; Mass spectrometry; Mass spectroscopy; Metabolism; Morphology; Oreochromis niloticus; Phosphatase; Ponds; Quinic acid; Serjania; Tilapia; Trimers; Zoology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-019-00622-9

The aim of this study was to assess the leaf aqueous extract composition of Serjania marginata and the effects of its inclusion on the diet of Nile tilapia ( Oreochromis niloticus ), with respect to the activity of digestive enzymes and enzymes associated with the metabolism of the liver and intestine and liver histopathology. Fish (initial mean weight, 54.36 ± 17.04 g) were divided into groups: fasting (without feeding), control (commercial feed), and treatment (commercial feed with leaf aqueous extract of Serjania marginata ), and in each aquarium, there were five individuals and the fish fed ad libitum for 15 days. Treatment fish had ingested on average 224.3 mg of extract/kg of fish/day. In the extract analysis by mass spectrometry, quercitrin, isoquercitrin, A-type proanthocyanidin trimer, and quinic acid were identified. In the enzymatic activity, fish from the treatment group showed higher level of alkaline phosphatase, while the hepatotoxic markers (AST and ALT) and levels of lipase, amylase, and nonspecific protease did not differ ( p  > 0.05). In liver histopathological analysis, it was observed that fish from the treatment showed normal structure, while abnormalities were associated with control (fibrosis, loss of cordonal architecture, vacuolated hepatocytes with nucleus displaced to the periphery) and fasting (reduction in hepatocyte size and sinusoidal space). The intestine histopathology evidenced that the extract favored the development of goblet cells and intestinal fold height. The results indicated that the leaf aqueous extract of S . marginata assists in the structural maintenance of the liver and intestine and stimulates intestinal alkaline phosphatase production in Nile tilapia, suggesting that the identified compounds act on the liver and intestine, showing hepatoprotective effects and stimulating intestinal digestion.