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Molecular Characterization and Tissue Expression of Common Tobacco (Nicotiana tabacum) Cadmium Resistance Protein 2 and Metal Tolerance Protein 4 Genes
The complete coding sequences of common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes were amplified by RT-PCR. The tobacco cadmium resistance protein 2 gene encodes a protein of 192 amino acids which shares high identity with the cadmium resistance protein 2 of four speci...
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Published in: | Cytology and genetics 2019-03, Vol.53 (2), p.169-177 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | The complete coding sequences of common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes were amplified by RT-PCR. The tobacco cadmium resistance protein 2 gene encodes a protein of 192 amino acids which shares high identity with the cadmium resistance protein 2 of four species—
nicotiana tomentosiformis
(99%), wood tobacco (97%),
capsicum annuum
(90%) and potato (86%). The tobacco metal tolerance protein 4 gene encodes a protein of 415 amino acids which shares high identity with the metal tolerance protein 4 of six species—
nicotiana tomentosiformis
(90%), wood tobacco (90%),
lycopersicon esculentum
(84%), potato (84%),
lycopersicon pennellii
(83%) and
capsicum annuum
(82%). Prediction of transmembrane helices showed that cadmium resistance protein 2 and metal tolerance protein 4 might be two transmembrane proteins. Phylogenetic analysis revealed that the common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes both have a closer genetic relationship with the wood tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes. Computer-assisted analysis showed that cadmium resistance protein 2 gene is structured in 4 exons and 3 introns and metal tolerance protein 4 gene consists of 7 exons and 6 introns. The gene expression profile analysis indicated that the common tobacco cadmium resistance protein 2 and metal tolerance protein 4 genes were highly expressed in root. |
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ISSN: | 0095-4527 1934-9440 |
DOI: | 10.3103/S009545271902004X |