Proteomic and Physiological Analyses of dl-Cyclopentane-1,2,3-triol-Treated Barley Under Drought Stress

This study was conducted to determine the effect of exogenous cyclitol ( dl -cyclopentane-1,2,3-triol) on barley ( Hordeum vulgare L.) during the alleviation of drought stress by using proteomics and physiological approaches. With the relative water content, water potential declined in barley depend...

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Bibliographic Details
Published in:Plant molecular biology reporter 2019-06, Vol.37 (3), p.237-251
Main Authors: Çevik, Sertan, Değer, Ayşin Güzel, Yıldızlı, Aytunç, Doğanyiğit, Neslihan, Gök, Ayşe, Ünyayar, Serpil
Format: Article
Language:English
Subjects:
Abscisic acid
Barley
Bioinformatics
Biomedical and Life Sciences
Biosynthesis
Cyclopentane
Drought
Drought resistance
Energy metabolism
Fluorescence
Levels
Life Sciences
Metabolism
Metabolomics
Moisture content
Original Article
Photosynthesis
Physiology
Plant Breeding/Biotechnology
Plant Sciences
Proline
Proteomics
Seedlings
Signal transduction
Stomata
Water content
Water potential
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Summary:This study was conducted to determine the effect of exogenous cyclitol ( dl -cyclopentane-1,2,3-triol) on barley ( Hordeum vulgare L.) during the alleviation of drought stress by using proteomics and physiological approaches. With the relative water content, water potential declined in barley depending on time under drought stress. This decline was lower in cyclitol-treated plants than in untreated plants. The stomatal pore width and open stoma number were higher in cyclitol-treated barley than in untreated plants under drought stress. The abscisic acid (ABA) and proline levels increased with the severity of drought in most treatments. The ABA level was lower in cyclitol-treated plants than in untreated plants under drought stress. Cyclitol treatments reduced O 2 . − fluorescence in leaf cells under drought stress. Total differentially expressed proteins were identified in cyclitol treatment under stressful and unstressful conditions. Photosynthesis (approx. 49%) and energy metabolism (approx. 27%) were upregulated by cyclitol treatment under drought. Moreover, CML42, acting as a negative ABA regulator, also increased by cyclitol treatment. This increase may be important in controlling stomata movements under drought stress. Our results suggest that exogenous cyclitol enhances the drought tolerance of barley seedlings by modulating the photosynthesis, energy pathway, biosynthesis, and signal transduction.
ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-019-01151-8