Effect of allopurinol on germ cell apoptosis following testicular ischemia–reperfusion injury in a rat

Recent evidence suggests that apoptosis is involved in germ cell loss following testicular ischemia-reperfusion (IR) injury. Allopurinol (Allo) is as a free radical scavenger which prevents tissue damage caused by reperfusion and oxygenation after ischemia; however, its effect on apoptosis in this t...

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Bibliographic Details
Published in:Pediatric surgery international 2008, Vol.24 (1), p.61-66
Main Authors: Sukhotnik, Igor, Meyer, Gil, Nativ, Ofer, Coran, Arnold G., Voskoboinik, Katya, Shiloni, Eitan, Mogilner, Jorge G.
Format: Article
Language:English
Subjects:
Allopurinol - therapeutic use
Animals
Apoptosis - drug effects
Disease Models, Animal
Free Radical Scavengers - therapeutic use
In Situ Nick-End Labeling
Male
Medicine
Medicine & Public Health
Original Article
Pediatric Surgery
Pediatrics
Rats
Rats, Sprague-Dawley
Reperfusion Injury - drug therapy
Reperfusion Injury - pathology
Spermatogenesis - drug effects
Spermatozoa - drug effects
Spermatozoa - pathology
Surgery
Testis - blood supply
Treatment Outcome
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Summary:Recent evidence suggests that apoptosis is involved in germ cell loss following testicular ischemia-reperfusion (IR) injury. Allopurinol (Allo) is as a free radical scavenger which prevents tissue damage caused by reperfusion and oxygenation after ischemia; however, its effect on apoptosis in this type of injury has not been studied. To examine the effect of allopurinol on germ cell apoptosis following testicular IR in a rat. Forty rats were divided randomly into 4 experimental groups of 10 rats each: group A (Sham)-Sham operated animals; group B (Sham-Allo)-Sham operated rats treated with allopurinol given PO (by gavage) at a dose of 200 mg/kg, once daily, immediately before and 24 h following operation; group C (IR)-rats underwent 90 min of unilateral testicular ischemia and 48 h of reperfusion; group D (IR-Allo)-rats underwent IR and were treated with allopurinol similar to group B. The ipsilateral and contralateral testes were harvested 48 h following operation. Johnsen’s criteria and the number of germinal cell layers were used to categorize spermatogenesis. TUNEL assay was used to determine germ cell apoptosis. Statistical analysis was performed using one-way ANOVA test, with P  
ISSN:0179-0358
1437-9813
DOI:10.1007/s00383-007-2042-3