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Effect of allopurinol on germ cell apoptosis following testicular ischemia–reperfusion injury in a rat
Recent evidence suggests that apoptosis is involved in germ cell loss following testicular ischemia-reperfusion (IR) injury. Allopurinol (Allo) is as a free radical scavenger which prevents tissue damage caused by reperfusion and oxygenation after ischemia; however, its effect on apoptosis in this t...
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| Published in: | Pediatric surgery international 2008, Vol.24 (1), p.61-66 |
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| creator | Sukhotnik, Igor Meyer, Gil Nativ, Ofer Coran, Arnold G. Voskoboinik, Katya Shiloni, Eitan Mogilner, Jorge G. |
| description | Recent evidence suggests that apoptosis is involved in germ cell loss following testicular ischemia-reperfusion (IR) injury. Allopurinol (Allo) is as a free radical scavenger which prevents tissue damage caused by reperfusion and oxygenation after ischemia; however, its effect on apoptosis in this type of injury has not been studied. To examine the effect of allopurinol on germ cell apoptosis following testicular IR in a rat. Forty rats were divided randomly into 4 experimental groups of 10 rats each: group A (Sham)-Sham operated animals; group B (Sham-Allo)-Sham operated rats treated with allopurinol given PO (by gavage) at a dose of 200 mg/kg, once daily, immediately before and 24 h following operation; group C (IR)-rats underwent 90 min of unilateral testicular ischemia and 48 h of reperfusion; group D (IR-Allo)-rats underwent IR and were treated with allopurinol similar to group B. The ipsilateral and contralateral testes were harvested 48 h following operation. Johnsen’s criteria and the number of germinal cell layers were used to categorize spermatogenesis. TUNEL assay was used to determine germ cell apoptosis. Statistical analysis was performed using one-way ANOVA test, with
P
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| doi_str_mv | 10.1007/s00383-007-2042-3 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_222700677</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1413103681</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-87556ced1abbb3a3df81e3a922e5c7ba0d32360bd5cc7ef9d26f7c46804881813</originalsourceid><addsrcrecordid>eNp1kE1OwzAQhS0EoqVwADbIYh8Y20nsLFFVfiQkNrCOHMduXSVxsBOh7rgDN-QkuEqlrljNaPy9N56H0DWBOwLA7wMAEyyJbUIhpQk7QXOSMp4UgrBTNAfCiwRYJmboIoQtAAiWF-doFuciIymZo83KGK0G7AyWTeP60dvONdh1eK19i5VuGix71w8u2ICNi8yX7dZ40GGwamykxzaojW6t_P3-8brX3ozBRr3ttqPfxYIl9nK4RGdGNkFfHeoCfTyu3pfPyevb08vy4TVRrBBDIniW5UrXRFZVxSSrjSCayYJSnSleSagZZTlUdaYU16aoaW64SnMBqRAknr1At5Nv793nGH9Zbt3ou7iypJRygJzzCJEJUt6F4LUpe29b6XclgXIfbTlFW-7bfbQli5qbg_FYtbo-Kg5ZRoBOQIhPXYzvuPl_1z9izIan</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>222700677</pqid></control><display><type>article</type><title>Effect of allopurinol on germ cell apoptosis following testicular ischemia–reperfusion injury in a rat</title><source>Springer Link</source><creator>Sukhotnik, Igor ; Meyer, Gil ; Nativ, Ofer ; Coran, Arnold G. ; Voskoboinik, Katya ; Shiloni, Eitan ; Mogilner, Jorge G.</creator><creatorcontrib>Sukhotnik, Igor ; Meyer, Gil ; Nativ, Ofer ; Coran, Arnold G. ; Voskoboinik, Katya ; Shiloni, Eitan ; Mogilner, Jorge G.</creatorcontrib><description><![CDATA[Recent evidence suggests that apoptosis is involved in germ cell loss following testicular ischemia-reperfusion (IR) injury. Allopurinol (Allo) is as a free radical scavenger which prevents tissue damage caused by reperfusion and oxygenation after ischemia; however, its effect on apoptosis in this type of injury has not been studied. To examine the effect of allopurinol on germ cell apoptosis following testicular IR in a rat. Forty rats were divided randomly into 4 experimental groups of 10 rats each: group A (Sham)-Sham operated animals; group B (Sham-Allo)-Sham operated rats treated with allopurinol given PO (by gavage) at a dose of 200 mg/kg, once daily, immediately before and 24 h following operation; group C (IR)-rats underwent 90 min of unilateral testicular ischemia and 48 h of reperfusion; group D (IR-Allo)-rats underwent IR and were treated with allopurinol similar to group B. The ipsilateral and contralateral testes were harvested 48 h following operation. Johnsen’s criteria and the number of germinal cell layers were used to categorize spermatogenesis. TUNEL assay was used to determine germ cell apoptosis. Statistical analysis was performed using one-way ANOVA test, with
P
< 0.05 considered statistically significant. Testicular ischemia in rats led to histological damage in the ipsilateral testis. In the contralateral testis minimal damage was observed. Treatment with allopurinol increased significantly Johnsen’s score in both the ischemic (7.3 ± 0.5 vs 5.6 ± 0.5,
P
< 0.05) and contralateral (8.9 ± 0.1 vs 8.3 ± 0.2,
P
< 0.05) testis, compared to IR-animals. Germ cell apoptosis in both the ischemic and the contralateral testis increased significantly after IR. Treatment with allopurinol resulted in a significant decrease in germ cell apoptosis in the ipsilateral testis, expressed as the number of positive tubules per 100 tubules (AI-1, (apoptotic index) threefold decrease,
P
< 0.005) and the number of apoptotic cells per 100 tubules (AI-2, fivefold decrease,
P
< 0.005) as well as a significant decrease in germ cell apoptosis in the contralateral testis (AI-1, 3.5-fold decrease,
P
< 0.05, AI-2- sixfold decrease,
P
< 0.005) compared to IR animals. In a rat model of testicular IR, treatment with allopurinol decreases germ cell apoptosis in both ischemic and contralateral testes and improves spermatogenesis.]]></description><identifier>ISSN: 0179-0358</identifier><identifier>EISSN: 1437-9813</identifier><identifier>DOI: 10.1007/s00383-007-2042-3</identifier><identifier>PMID: 17985141</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Allopurinol - therapeutic use ; Animals ; Apoptosis - drug effects ; Disease Models, Animal ; Free Radical Scavengers - therapeutic use ; In Situ Nick-End Labeling ; Male ; Medicine ; Medicine & Public Health ; Original Article ; Pediatric Surgery ; Pediatrics ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - drug therapy ; Reperfusion Injury - pathology ; Spermatogenesis - drug effects ; Spermatozoa - drug effects ; Spermatozoa - pathology ; Surgery ; Testis - blood supply ; Treatment Outcome</subject><ispartof>Pediatric surgery international, 2008, Vol.24 (1), p.61-66</ispartof><rights>Springer-Verlag 2007</rights><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-87556ced1abbb3a3df81e3a922e5c7ba0d32360bd5cc7ef9d26f7c46804881813</citedby><cites>FETCH-LOGICAL-c398t-87556ced1abbb3a3df81e3a922e5c7ba0d32360bd5cc7ef9d26f7c46804881813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17985141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sukhotnik, Igor</creatorcontrib><creatorcontrib>Meyer, Gil</creatorcontrib><creatorcontrib>Nativ, Ofer</creatorcontrib><creatorcontrib>Coran, Arnold G.</creatorcontrib><creatorcontrib>Voskoboinik, Katya</creatorcontrib><creatorcontrib>Shiloni, Eitan</creatorcontrib><creatorcontrib>Mogilner, Jorge G.</creatorcontrib><title>Effect of allopurinol on germ cell apoptosis following testicular ischemia–reperfusion injury in a rat</title><title>Pediatric surgery international</title><addtitle>Pediatr Surg Int</addtitle><addtitle>Pediatr Surg Int</addtitle><description><![CDATA[Recent evidence suggests that apoptosis is involved in germ cell loss following testicular ischemia-reperfusion (IR) injury. Allopurinol (Allo) is as a free radical scavenger which prevents tissue damage caused by reperfusion and oxygenation after ischemia; however, its effect on apoptosis in this type of injury has not been studied. To examine the effect of allopurinol on germ cell apoptosis following testicular IR in a rat. Forty rats were divided randomly into 4 experimental groups of 10 rats each: group A (Sham)-Sham operated animals; group B (Sham-Allo)-Sham operated rats treated with allopurinol given PO (by gavage) at a dose of 200 mg/kg, once daily, immediately before and 24 h following operation; group C (IR)-rats underwent 90 min of unilateral testicular ischemia and 48 h of reperfusion; group D (IR-Allo)-rats underwent IR and were treated with allopurinol similar to group B. The ipsilateral and contralateral testes were harvested 48 h following operation. Johnsen’s criteria and the number of germinal cell layers were used to categorize spermatogenesis. TUNEL assay was used to determine germ cell apoptosis. Statistical analysis was performed using one-way ANOVA test, with
P
< 0.05 considered statistically significant. Testicular ischemia in rats led to histological damage in the ipsilateral testis. In the contralateral testis minimal damage was observed. Treatment with allopurinol increased significantly Johnsen’s score in both the ischemic (7.3 ± 0.5 vs 5.6 ± 0.5,
P
< 0.05) and contralateral (8.9 ± 0.1 vs 8.3 ± 0.2,
P
< 0.05) testis, compared to IR-animals. Germ cell apoptosis in both the ischemic and the contralateral testis increased significantly after IR. Treatment with allopurinol resulted in a significant decrease in germ cell apoptosis in the ipsilateral testis, expressed as the number of positive tubules per 100 tubules (AI-1, (apoptotic index) threefold decrease,
P
< 0.005) and the number of apoptotic cells per 100 tubules (AI-2, fivefold decrease,
P
< 0.005) as well as a significant decrease in germ cell apoptosis in the contralateral testis (AI-1, 3.5-fold decrease,
P
< 0.05, AI-2- sixfold decrease,
P
< 0.005) compared to IR animals. In a rat model of testicular IR, treatment with allopurinol decreases germ cell apoptosis in both ischemic and contralateral testes and improves spermatogenesis.]]></description><subject>Allopurinol - therapeutic use</subject><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Disease Models, Animal</subject><subject>Free Radical Scavengers - therapeutic use</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Article</subject><subject>Pediatric Surgery</subject><subject>Pediatrics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - drug therapy</subject><subject>Reperfusion Injury - pathology</subject><subject>Spermatogenesis - drug effects</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - pathology</subject><subject>Surgery</subject><subject>Testis - blood supply</subject><subject>Treatment Outcome</subject><issn>0179-0358</issn><issn>1437-9813</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp1kE1OwzAQhS0EoqVwADbIYh8Y20nsLFFVfiQkNrCOHMduXSVxsBOh7rgDN-QkuEqlrljNaPy9N56H0DWBOwLA7wMAEyyJbUIhpQk7QXOSMp4UgrBTNAfCiwRYJmboIoQtAAiWF-doFuciIymZo83KGK0G7AyWTeP60dvONdh1eK19i5VuGix71w8u2ICNi8yX7dZ40GGwamykxzaojW6t_P3-8brX3ozBRr3ttqPfxYIl9nK4RGdGNkFfHeoCfTyu3pfPyevb08vy4TVRrBBDIniW5UrXRFZVxSSrjSCayYJSnSleSagZZTlUdaYU16aoaW64SnMBqRAknr1At5Nv793nGH9Zbt3ou7iypJRygJzzCJEJUt6F4LUpe29b6XclgXIfbTlFW-7bfbQli5qbg_FYtbo-Kg5ZRoBOQIhPXYzvuPl_1z9izIan</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Sukhotnik, Igor</creator><creator>Meyer, Gil</creator><creator>Nativ, Ofer</creator><creator>Coran, Arnold G.</creator><creator>Voskoboinik, Katya</creator><creator>Shiloni, Eitan</creator><creator>Mogilner, Jorge G.</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>2008</creationdate><title>Effect of allopurinol on germ cell apoptosis following testicular ischemia–reperfusion injury in a rat</title><author>Sukhotnik, Igor ; Meyer, Gil ; Nativ, Ofer ; Coran, Arnold G. ; Voskoboinik, Katya ; Shiloni, Eitan ; Mogilner, Jorge G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-87556ced1abbb3a3df81e3a922e5c7ba0d32360bd5cc7ef9d26f7c46804881813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Allopurinol - therapeutic use</topic><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Disease Models, Animal</topic><topic>Free Radical Scavengers - therapeutic use</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Article</topic><topic>Pediatric Surgery</topic><topic>Pediatrics</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - drug therapy</topic><topic>Reperfusion Injury - pathology</topic><topic>Spermatogenesis - drug effects</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - pathology</topic><topic>Surgery</topic><topic>Testis - blood supply</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sukhotnik, Igor</creatorcontrib><creatorcontrib>Meyer, Gil</creatorcontrib><creatorcontrib>Nativ, Ofer</creatorcontrib><creatorcontrib>Coran, Arnold G.</creatorcontrib><creatorcontrib>Voskoboinik, Katya</creatorcontrib><creatorcontrib>Shiloni, Eitan</creatorcontrib><creatorcontrib>Mogilner, Jorge G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Family Health Database (Proquest)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Pediatric surgery international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sukhotnik, Igor</au><au>Meyer, Gil</au><au>Nativ, Ofer</au><au>Coran, Arnold G.</au><au>Voskoboinik, Katya</au><au>Shiloni, Eitan</au><au>Mogilner, Jorge G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of allopurinol on germ cell apoptosis following testicular ischemia–reperfusion injury in a rat</atitle><jtitle>Pediatric surgery international</jtitle><stitle>Pediatr Surg Int</stitle><addtitle>Pediatr Surg Int</addtitle><date>2008</date><risdate>2008</risdate><volume>24</volume><issue>1</issue><spage>61</spage><epage>66</epage><pages>61-66</pages><issn>0179-0358</issn><eissn>1437-9813</eissn><abstract><![CDATA[Recent evidence suggests that apoptosis is involved in germ cell loss following testicular ischemia-reperfusion (IR) injury. Allopurinol (Allo) is as a free radical scavenger which prevents tissue damage caused by reperfusion and oxygenation after ischemia; however, its effect on apoptosis in this type of injury has not been studied. To examine the effect of allopurinol on germ cell apoptosis following testicular IR in a rat. Forty rats were divided randomly into 4 experimental groups of 10 rats each: group A (Sham)-Sham operated animals; group B (Sham-Allo)-Sham operated rats treated with allopurinol given PO (by gavage) at a dose of 200 mg/kg, once daily, immediately before and 24 h following operation; group C (IR)-rats underwent 90 min of unilateral testicular ischemia and 48 h of reperfusion; group D (IR-Allo)-rats underwent IR and were treated with allopurinol similar to group B. The ipsilateral and contralateral testes were harvested 48 h following operation. Johnsen’s criteria and the number of germinal cell layers were used to categorize spermatogenesis. TUNEL assay was used to determine germ cell apoptosis. Statistical analysis was performed using one-way ANOVA test, with
P
< 0.05 considered statistically significant. Testicular ischemia in rats led to histological damage in the ipsilateral testis. In the contralateral testis minimal damage was observed. Treatment with allopurinol increased significantly Johnsen’s score in both the ischemic (7.3 ± 0.5 vs 5.6 ± 0.5,
P
< 0.05) and contralateral (8.9 ± 0.1 vs 8.3 ± 0.2,
P
< 0.05) testis, compared to IR-animals. Germ cell apoptosis in both the ischemic and the contralateral testis increased significantly after IR. Treatment with allopurinol resulted in a significant decrease in germ cell apoptosis in the ipsilateral testis, expressed as the number of positive tubules per 100 tubules (AI-1, (apoptotic index) threefold decrease,
P
< 0.005) and the number of apoptotic cells per 100 tubules (AI-2, fivefold decrease,
P
< 0.005) as well as a significant decrease in germ cell apoptosis in the contralateral testis (AI-1, 3.5-fold decrease,
P
< 0.05, AI-2- sixfold decrease,
P
< 0.005) compared to IR animals. In a rat model of testicular IR, treatment with allopurinol decreases germ cell apoptosis in both ischemic and contralateral testes and improves spermatogenesis.]]></abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>17985141</pmid><doi>10.1007/s00383-007-2042-3</doi><tpages>6</tpages></addata></record> |
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| subjects | Allopurinol - therapeutic use Animals Apoptosis - drug effects Disease Models, Animal Free Radical Scavengers - therapeutic use In Situ Nick-End Labeling Male Medicine Medicine & Public Health Original Article Pediatric Surgery Pediatrics Rats Rats, Sprague-Dawley Reperfusion Injury - drug therapy Reperfusion Injury - pathology Spermatogenesis - drug effects Spermatozoa - drug effects Spermatozoa - pathology Surgery Testis - blood supply Treatment Outcome |
| title | Effect of allopurinol on germ cell apoptosis following testicular ischemia–reperfusion injury in a rat |
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