Decrease of parafollicular thyroid C-cells in experimental esophageal atresia: further evidence of a neural crest pathogenic pathway

Adriamycin-induced experimental esophageal atresia (EA) is often associated with malformations of neural crest (NC) origin, such as abnormal pharyngeal pouch derivatives like the thymus and the parathyroids. The aim of the present study was to examine whether NC-derived thyroid C-cells were abnormal...

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Bibliographic Details
Published in:Pediatric surgery international 2005-03, Vol.21 (3), p.175-179
Main Authors: Martinez, L, Ceano-Vivas, M De, Gonzalez-Reyes, S, Hernandez, F, Fernandez-Dumont, V, Calonge, W M, Ruiz, E, Rodriguez, J I, Tovar, J A
Format: Article
Language:English
Subjects:
Animals
Antibiotics, Antineoplastic - toxicity
Antibodies - immunology
Calcitonin - immunology
Calcitonin - metabolism
Cell Count
Disease Models, Animal
Doxorubicin - toxicity
Esophageal Atresia - chemically induced
Esophageal Atresia - complications
Esophageal Atresia - pathology
Female
Gestational Age
Immunohistochemistry
Neural Crest - abnormalities
Neural Crest - drug effects
Neural Crest - metabolism
Pregnancy
Random Allocation
Rats
Rats, Sprague-Dawley
Thyroid Diseases - etiology
Thyroid Diseases - metabolism
Thyroid Diseases - pathology
Thyroid Gland - abnormalities
Thyroid Gland - drug effects
Thyroid Gland - pathology
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Summary:Adriamycin-induced experimental esophageal atresia (EA) is often associated with malformations of neural crest (NC) origin, such as abnormal pharyngeal pouch derivatives like the thymus and the parathyroids. The aim of the present study was to examine whether NC-derived thyroid C-cells were abnormal in a rat model. Pregnant rats received intraperitoneally either 2 mg/kg Adriamycin (EA) or vehicle (controls) on days 8 and 9 of gestation. Fetuses were recovered on day 21, and blocks including the trachea and thyroid were fixed in formalin, coronally sectioned at 3-mum widths, and stained with standard hematoxylin and eosin until the largest area of thyroid was reached. From this point on, the 1st, 10th, and 20th slices were immunohistochemically stained with anti-calcitonin antibody. Positively-stained cells in each section of the gland were counted using a computer-assisted image analysis method, and the results were averaged. The distribution of the cells within the gland was assessed as well. Comparisons between EA and control rats were made by nonparametric tests with a significance threshold of p
ISSN:0179-0358
1437-9813
DOI:10.1007/s00383-004-1315-3