Performance Characteristics of Seven Neuron-Specific Enolase Assays

Background/Aims: The determination of neuron-specific enolase (NSE) is relatively frequently requested in the differential diagnosis of small-cell lung carcinoma and non-small-cell lung carcinoma. The individual results of different immunoassays are often not comparable, which has been confirmed by...

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Published in:Tumor biology 2007-01, Vol.28 (2), p.84-92
Main Authors: Stern, Petr, Bartos, Vladimir, Uhrova, Jana, Bezdickova, Drahomira, Vanickova, Zdislava, Tichy, Vojtech, Pelinkova, Kveta, Prusa, Richard, Zima, Tomas
Format: Article
Language:English
Subjects:
Adenocarcinoma - blood
Adenocarcinoma - enzymology
Biological Assay
Biomarkers, Tumor - metabolism
Carcinoma, Large Cell - blood
Carcinoma, Large Cell - enzymology
Carcinoma, Non-Small-Cell Lung - blood
Carcinoma, Non-Small-Cell Lung - enzymology
Carcinoma, Small Cell - blood
Carcinoma, Small Cell - enzymology
Carcinoma, Squamous Cell - blood
Carcinoma, Squamous Cell - enzymology
Case-Control Studies
Enzyme-Linked Immunosorbent Assay
Humans
Lung Neoplasms - blood
Lung Neoplasms - enzymology
Phosphopyruvate Hydratase - blood
Research Article
Sensitivity and Specificity
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Summary:Background/Aims: The determination of neuron-specific enolase (NSE) is relatively frequently requested in the differential diagnosis of small-cell lung carcinoma and non-small-cell lung carcinoma. The individual results of different immunoassays are often not comparable, which has been confirmed by long-term external quality assessments. In this study, we assessed the possible sources of these differences. Methods: More than 3,000 NSE analyses were performed using seven different immunoassays: DELFIA (PerkinElmer), Elecsys 2010 or Modular Analytics E 170 (Roche), Kryptor (B.R.A.H.M.S.), the enzyme-linked immunosorbent assay DRG and three assays based on immunoradiometric assays (DiaSorin, Immunotech and Schering-CIS). The following parameters were evaluated: precision profile of the individual methods, linearity on dilution and modified recovery, comparability and discrimination of immunoassays, sensitivity, and specificity. Results: There were differences in the correlation of values of certain low-concentration specimens. Some assays correlate well while others do not (up to fivefold difference), especially in the case of controls prepared synthetically. Therefore, the current non-standardized preparation of controls is questionable in our opinion. In the cutoff range, the difference in the results of native samples did not exceed its double value. The variation in values >100 µg/l obtained with different assays is
ISSN:1010-4283
1423-0380
DOI:10.1159/000098441