Reactivity of Anti-PSA Monoclonal Antibodies with Recombinant Human Kallikrein-2

Seventy-nine monoclonal antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for their reactivity with recombinant human kallikrein-2 (rhK2). A sandwich immunofluorometric assay using polyclonal anti-prostate-specific antigen (PSA) antiserum-coated plates was used to capture rhK2 and subs...

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Bibliographic Details
Published in:Tumor biology 1999-01, Vol.20 (Suppl 1), p.35-37
Main Authors: Leinonen, J., Leinimaa, M., Zhang, W.-M., Piironen, T., Pettersson, K., Lilja, H., Dowell, B., Stenman, U.-H.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Basic Medicine
Cross Reactions
Fluoroimmunoassay
Human kallikrein-2
Humans
ISOBM
Läkemedelskemi
Medical and Health Sciences
Medicin och hälsovetenskap
Medicinal Chemistry
Medicinska och farmaceutiska grundvetenskaper
Monoclonal antibodies
Prostate-specific antigen
Prostate-Specific Antigen - immunology
Recombinant kallikrein
Recombinant Proteins - immunology
TD-3
Tissue Kallikreins - immunology
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Summary:Seventy-nine monoclonal antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for their reactivity with recombinant human kallikrein-2 (rhK2). A sandwich immunofluorometric assay using polyclonal anti-prostate-specific antigen (PSA) antiserum-coated plates was used to capture rhK2 and subsequently the test antibody. The response of each test antibody was compared with 3 reference antibodies (H50, H117 and 5E4) known to react with hK2. Nine antibodies from the workshop panel failed to react with purified PSA and rhK2 in this assay and were subsequently excluded. From the remaining panel of antibodies, 11/70 showed strong reactivity with rhK2, 9/70 showed weak reactivity with rhK2, while 50/70 antibodies did not react with rhK2 in this assay format. All antibodies binding to rhK2 recognized both free and complexed PSA.
ISSN:1010-4283
1423-0380
DOI:10.1159/000056528