Мicroscopic investigations (LM, TEM and SEM) and identification of Chlorella isolate R-06/2 from extreme habitat in Bulgaria with a strong biological activity and resistance to environmental stress factors

• Published in: Biotechnology, biotechnological equipment biotechnological equipment, 2015-05, Vol.29 (3), p.536-540
• Main Authors: Gärtner, Georg, Uzunov, Blagoy, Ingolic, Elisabeth, Kofler, Werner, Gacheva, Gergana, Pilarski, Plamen, Zagorchev, Lyuben, Odjakova, Mariela, Stoyneva, Maya
• Format: Article
• Language: English
• Subjects: Biological activity; Cell morphology; cell wall; Cell walls; Chlorella; Cytology; Electron microscopy; Environmental stress; extremophilic alga; Glucosamine; green algae; Light microscopy; Microscopy; Morphology; Optical microscopy; Reproduction (biology); Resistance factors; Scanning electron microscopy; Starch; thermophilic alga; Transmission electron microscopy; Ultrastructure; ultrastructure, pyrenoid
• ISSN: 1310-2818; 1314-3530
• DOI: 10.1080/13102818.2015.1013283

An extremophilic Chlorella strain R-06/2, isolated from a geothermal spring (+42 °C) in the region of Rupite village (SW Bulgaria), was investigated for species identification. This was done by observation of the cell morphology, reproduction and ultrastructure by light microscopy, scanning electron microscopy and transmission electron microscopy, and by investigation of the cell-wall chemistry. The pyrenoid ultrastructure with a double-layered thylakoid traversing the matrix, the shape of the starch envelope, as well as the cell wall, composed of glucosamine and developed around young autospores, were the features that allowed us to classify the thermophilic strain Chlorella R-06/2 as Chlorella vulgaris Beijerinck 1890.