Effect of Short-term Tumour Necrosis Factor-alpha (TNF-α) -stimulation on the Growth and Differentiation of MC3T3-E1 Osteoblast-like Cells

Tumor necrosis factor-alpha (TNF-α) is an inflammatory cytokine known to cause bone resorption, swelling and edema during tissue organization. Conversely, TNF-α has also been shown to participate in tissue regeneration during the wound healing process. We have previously investigated the effects of...

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Bibliographic Details
Published in:Journal of Hard Tissue Biology 2018/07/01, Vol.27(3), pp.213-218
Main Authors: Inoue, Miho, Naritani, Mio, Raju, Resmi, Miyagi, Mayu, Oshima, Masamitsu, Inoue, Masahisa, Matsuka, Yoshizo
Format: Article
Language:English
Subjects:
Biocompatibility
Bone resorption
Cell differentiation
Cell growth
Cell proliferation
Dental pulp
Differentiation (biology)
Edema
Fibroblasts
Inflammation
Odontoblasts
Osteoblast-like cell (MC3T3-E1)
Osteoblastogenesis
Regeneration
Short term
Stem cells
Stimulation
Tissue engineering
TNF inhibitors
TNF-α
Tumor necrosis factor-TNF
Tumor necrosis factor-α
Tumors
Wound healing
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Summary:Tumor necrosis factor-alpha (TNF-α) is an inflammatory cytokine known to cause bone resorption, swelling and edema during tissue organization. Conversely, TNF-α has also been shown to participate in tissue regeneration during the wound healing process. We have previously investigated the effects of TNF-α on human dental pulp cell differentiation. Dental pulp cells are composed of different cell types including primary odontoblasts and fibroblasts. We determined that the ratio of stem cells within the pulp cell population was increased following short-term stimulation with TNF-α. The aim of this study therefore was to investigate the effect of short-term stimulation with TNF-α on osteoblast-like MC3T3-E1 cell growth and differentiation. MC3T3-E1 cells were cultured in standard growth medium and on reaching sub-confluence were exposed to recombinant TNF-α (10 and 100 ng/ml) for 2 days prior to assessing their cell proliferation and differentiation properties in comparison to non-stimulated MC3T3-E1 cells (control). Although no significant differences in cell proliferation were observed between the TNF-α-stimulated and control groups, cell differentiation was delayed in the TNF-α-stimulated groups. In summary, short-term stimulation of cultured MC3T3-E1 cells with TNF-α had only minimal effect on their growth and differentiation.
ISSN:1341-7649
1880-828X
DOI:10.2485/jhtb.27.213