Biocontrol of Botrytis cinerea by chitin-based cultures of Paenibacillus elgii HOA73

The chitinase-producing bacterium, Paenibacillus elgii HOA73, is a biocontrol agent that limits the damage caused to plants by microbial pathogens, insects, and nematodes. However, the mechanisms involved in the biocontrol of plant diseases by HOA73 have not been determined. The objective of this st...

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Bibliographic Details
Published in:European journal of plant pathology 2019-09, Vol.155 (1), p.253-263
Main Authors: Kim, Young Cheol, Hur, Jin Young, Park, Seur Kee
Format: Article
Language:English
Subjects:
Agriculture
Antifungal activity
Bacteria
Biological control
Biomedical and Life Sciences
Botrytis cinerea
Cell culture
Chelation
Chitin
Chitinase
Conidia
Ecology
Fungicides
Germination
Grey mold
Insects
Integrated control
Life Sciences
Lipase
Microorganisms
Mold
Nematodes
Oligomers
Paenibacillus
Pathogens
Plant diseases
Plant Pathology
Plant Sciences
Plants (botany)
Siderophores
Tomatoes
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Summary:The chitinase-producing bacterium, Paenibacillus elgii HOA73, is a biocontrol agent that limits the damage caused to plants by microbial pathogens, insects, and nematodes. However, the mechanisms involved in the biocontrol of plant diseases by HOA73 have not been determined. The objective of this study was to elucidate the role of extracellular chitinase obtained from isolate HOA73 in the control of the fungal pathogen Botrytis cinerea , the causative agent of gray mold in tomato. The HOA73 strain grew efficiently in a chitin-containing broth and produced chitin oligomers through chitinase activity; protease, lipase, and Fe-chelating siderophores were also secreted by the bacterium. Cultures containing intact bacteria inhibited B. cinerea conidia germination to a greater extent than did the bacterial cells alone or the cell-free culture supernatant. The antifungal activity increased with culture age and was heat-sensitive because of chitinase-mediated production of long-chain chitin oligomers. The biocontrol efficacy of undiluted bacterial cultures against gray mold in tomato was comparable to that of a standard fungicide. This study demonstrated that P. elgii HOA73 bacterial cultures grown on chitin-based minimal medium may be an effective formulation for the integrated control of gray mold.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-019-01768-1