Delivery of Survivin siRNA Using Cationic Diphenylalanine Vesicles

Small interfering RNA(siRNA) has been proved to be a powerful tool for silencing target gene in cells, raising the possibility that siRNA can be employed as a therapy for treating cancers and other genetic diseases. However, siRNA transfection has the limitation due to the difficulty in the delivery...

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Bibliographic Details
Published in:Chemical research in Chinese universities 2019-06, Vol.35 (3), p.434-439
Main Authors: Guan, Shuwen, Yu, Xiaoxuan, Li, Junyang, Xu, Heng, Han, Wenzhao, Shi, Guannan, Xu, Jia, Wang, Liping
Format: Article
Language:English
Subjects:
Analytical Chemistry
Apoptosis
Biocompatibility
Cancer
Cations
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Inorganic Chemistry
Microscopy
Morphology
Organic Chemistry
Ovarian cancer
Physical Chemistry
Scanning electron microscopy
Scanning microscopy
Survival
Therapy
Vesicles
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Summary:Small interfering RNA(siRNA) has been proved to be a powerful tool for silencing target gene in cells, raising the possibility that siRNA can be employed as a therapy for treating cancers and other genetic diseases. However, siRNA transfection has the limitation due to the difficulty in the delivery of siRNA to target cells and tissues. To explore an efficient biocompatible siRNA delivery system, cationic diphenylalanine vesicles(CDPVs) were constructed to transfer survivin siRNA to human ovarian cancer cells. The morphology of CDPVs was characterized by scanning electron microscopy(SEM) and the distribution of survivin siRNA was characterized by confocal laser scanning microscopy, which reveal that diphenylalanine and the survivin siRNA were successfully co-delivered. After co-incubation for 48 h, the CDPVs/siRNA exhibited enhanced tumor cell growth inhabitation and apoptosis inducted in human SK-OV-3 ovarian carcinoma cells. Overall, CDPVs is an efficient siRNA delivery system and has a promising prospect for cancer therapy.
ISSN:1005-9040
2210-3171
DOI:10.1007/s40242-019-8184-8