Development of a Universal Fluorescent Probe for Gram‐Positive Bacteria

• Published in: Angewandte Chemie 2019-06, Vol.131 (25), p.8514-8519
• Main Authors: Kwon, Haw‐Young, Liu, Xiao, Choi, Eun Gyeong, Lee, Jung Yeol, Choi, So‐Young, Kim, Jun‐Young, Wang, Lu, Park, Sung‐Jin, Kim, Beomsue, Lee, Yong‐An, Kim, Jong‐Jin, Kang, Nam Young, Chang, Young‐Tae
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial infections; Biologische Bildgebung; Borsäure; Chemistry; Communities; Fluorescent indicators; Fluoreszenzsonden; Gram-positive Bakterien; Infections; Keratitis; Selectivity; Sludge; Struktur-Aktivitäts-Beziehungen; Wastewater
• ISSN: 0044-8249; 1521-3757
• DOI: 10.1002/ange.201902537

The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram‐positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect a Gram‐positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented research tool for the study of dynamic bacterial communities and for clinical application. BacGO, eine neuartige Gram‐positive Bakteriensonde, wurde aus einer Bibliothek fluoreszierender Moleküle mit Borsäuremotiv, das an Peptidoglykan in der Gram‐positiven Bakterienzellwand bindet, entwickelt. BacGO kann zur Identifizierung Gram‐positiver Bakterien in diversen, hochkomplexen Proben eingesetzt werden und ist eine attraktive Alternative zur Gram‐Färbung.