SEMISYNTHESIS OF PROTEINS BY EXPRESSED PROTEIN LIGATION

Expressed protein ligation (EPL) is a protein engineering approach that allows recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined together. The approach makes the primary structure of most proteins accessible to the tools of synthetic organic chemistry, enablin...

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Bibliographic Details
Published in:Annual review of biochemistry 2003-01, Vol.72 (1), p.249-289
Main Author: Muir, Tom W
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - chemistry
Biochemistry
chemical ligation
Fluorescent Dyes - chemistry
inteins
Models, Molecular
Peptide Fragments - chemistry
Protein Biosynthesis
protein engineering
Protein Engineering - methods
Protein Engineering - trends
Protein Processing, Post-Translational
Protein Splicing
Proteins
Proteins - chemical synthesis
Proteins - genetics
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemical synthesis
Recombinant Proteins - genetics
semisynthesis
Stable isotopes
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Description
Summary:Expressed protein ligation (EPL) is a protein engineering approach that allows recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined together. The approach makes the primary structure of most proteins accessible to the tools of synthetic organic chemistry, enabling the covalent structure of proteins to be modified in an unprecedented fashion. The ability to incorporate noncoded amino acids, biophysical probes, and stable isotopes into specific locations within proteins provides research tools to peer into the inner workings of these molecules. In this review I discuss the development of this technology, its broad application to biological systems, and its possible role in the area of proteomics.
ISSN:0066-4154
1545-4509
DOI:10.1146/annurev.biochem.72.121801.161900