Mechanistic insight into the endophytic fungus mediated synthesis of protein capped ZnO nanoparticles

[Display omitted] •Zinc acetate tolerant endophytic fungi C. geniculatus from N. foetida was used for synthesis of ZnO nanoparticles.•VIS, FTIR, Fluorescence spectroscopy, SDS-PAGE analysis confirmed the role of extracellular proteins in ZnO NPs synthesis.•The biogenic ZnO nanoparticles were found t...

Full description

Saved in:
Bibliographic Details
Published in:Materials science & engineering. B, Solid-state materials for advanced technology Solid-state materials for advanced technology, 2019-04, Vol.243, p.214-221
Main Authors: Kadam, Vrushali Vinayak, Ettiyappan, Jagadeeshbabu Ponnan, Mohan Balakrishnan, Raj
Format: Article
Language:English
Subjects:
Biosynthesis
Capping
Capping protein
Cochliobolus geniculatus
Endophytic fungi
Energy transmission
Fluorescence
Fungi
Mycelial free filtrate
Nanoparticles
Proteins
Spectrum analysis
Synthesis
Transmission electron microscopy
Zinc oxide
ZnO nanoparticles
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] •Zinc acetate tolerant endophytic fungi C. geniculatus from N. foetida was used for synthesis of ZnO nanoparticles.•VIS, FTIR, Fluorescence spectroscopy, SDS-PAGE analysis confirmed the role of extracellular proteins in ZnO NPs synthesis.•The biogenic ZnO nanoparticles were found to be in hexagonal phase, quasi spherical with 2–6 nm in size and stable.•The mechanism of protein capped ZnO nanoparticles is also postulated in the present work. Present investigation is focused on the development of environment friendly protocol for ZnO nanoparticles synthesis using mycelial free filtrate of unexplored endophytic fungus Cochliobolus geniculatus. Zinc metal tolerant endophyte was isolated from leaves of Nothapodytes foetida and identified based on rDNA internal transcribed spacer region sequence. Surface plasmon resonance examined by UV–VIS spectroscopy analysis confirmed the synthesis of ZnO nanoparticles; exhibiting a band gap of 3.28 eV. The obtained nanoparticles were characterized by energy dispersive X-ray (EDX), high resolution transmission electron microscopy and XRD analysis. The synthesized ZnO nanoparticles were found to be polydispersed and well distributed deprived of agglomeration, crystalline, quasi spherical particles exhibiting a narrow range distribution. Fluorescence, FTIR and UV–VIS spectra depicted presence of extracellular proteins which was further confirmed by SDS-PAGE study that displayed occurrence of 52 kDa and 58 kDa protein bands involved in the synthesis of ZnO nanoparticles and further act as capping material.
ISSN:0921-5107
1873-4944
DOI:10.1016/j.mseb.2019.04.017