P22 The effects of storage method, temperature and extraction kits on the human milk microbiota

Human breast milk is the optimum feeding regime for new born infants. In terms of composition, breast milk contains the required nutrients and bioactive compounds (e.g. oligosaccharides, lactoferrin, secretory antibodies, immune cells, CD14, regulatory cytokines) to support the growth and immunity o...

Full description

Saved in:
Bibliographic Details
Published in:Archives of disease in childhood 2019-06, Vol.104 (Suppl 3), p.A164
Main Authors: Lyons, Katríona, Fouhy, Fiona, Shea, Carol-Anne O’, Anthony Ryan, C, Paul Ross, R, Kelly, Alan, Stanton, Catherine
Format: Article
Language:English
Subjects:
Baby foods
Bioactive compounds
Breast milk
Breastfeeding & lactation
CD14 antigen
Childrens health
Cold storage
Cytokines
Immune system
Infants
Lactoferrin
Microbiomes
Microbiota
Next-generation sequencing
Nutrients
Oligosaccharides
Pediatrics
Storage
Storage conditions
Young Children
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human breast milk is the optimum feeding regime for new born infants. In terms of composition, breast milk contains the required nutrients and bioactive compounds (e.g. oligosaccharides, lactoferrin, secretory antibodies, immune cells, CD14, regulatory cytokines) to support the growth and immunity of the developing infant. Until recently breast milk was considered to be a sterile fluid, however, advances in research has revealed that breast milk provides home to an array of bacterial species, which may provide various health benefits such as promoting gut colonisation, defence against pathogens and maturation of the immune system, an essential aspect of infant health.Next generation sequencing has enabled detailed insights into the complex microbial ecosystem of breast milk, however authenticity of the microbiome is subject to many factors such as sample collection, suitable storage and extraction method of the biological sample. While cold storage (4°C) immediately after sample collection until DNA extraction is optimal, other storage conditions need to be investigated for their efficacy when this option is not feasible. This study aims to investigate how different storage methods, temperatures additives and extraction techniques influence the human milk microbiome. Overall, 16S compositional sequencing analysis revealed no significant differences from either fresh or frozen samples.
ISSN:0003-9888
1468-2044
DOI:10.1136/archdischild-2019-epa.378