P327 Selective screening for celiac disease in children with abdominal pain

• Published in: Archives of disease in childhood 2019-06, Vol.104 (Suppl 3), p.A288
• Main Authors: Novikova, Valeria, Shapovalova, Natalia, Revnova, Maria, Lapin, Sergei, Kalinina, Elena
• Format: Article
• Language: English
• Subjects: Abdomen; Autoimmune diseases; Celiac disease; Children; Childrens health; Diabetes mellitus; Diabetes mellitus (insulin dependent); DQA1 protein; Enzyme immunoassay; Enzyme-linked immunosorbent assay; Epidemiology; Gliadin; Histocompatibility antigen HLA; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Pain; Pediatrics; Peptides; Polymerase chain reaction; Population studies; Risk groups; Thyroiditis; Transglutaminase 2
• ISSN: 0003-9888; 1468-2044
• DOI: 10.1136/archdischild-2019-epa.676

IntroductionModern epidemiologic data reveal that the prevalence of celiac disease (CD) is approximately 1% in Europe population. Statistics varies considerably in different countries. The latest Russian clinical recommendations ‘Celiac disease in children’ published in 2016, suggest the screening for CD only among children with type 1 diabetes mellitus and autoimmune thyroiditis. However, there are no population-based studies and data about prevalence of CD in average-risk and high-risk groups in Russia.AimTo determine prevalence of CD in children with abdominal pain.MethodsTissue transglutaminase antibodies (anti-tTG) of IgA, IgM and IgG classes were detected by quick celiac test «BIOHIT», based on immunochromatographic method. 500 children with abdominal pain, aged 3 – 17 years, out patients of the Second diagnostic center in St.Petersburg were examined using the quick-test. All positive for CD children underwent histological, genetic and serologic tests, as well as in 20 randomly selected children, who didn’t test positive. Antibodies to deamidated gliadin peptides (anti-DGPs) and anti-tTG were investigated by enzyme immunoassay (ELISA). The study patients were genotyped for HLA-DQB1and DQA1 alleles using real-time polymerase chain reaction.ResultsThe test detected 6 positive results (5 females with an average age of 12±2,1 years and one 10 years old boy) in group with abdominal pain. CD in all this subjects was diagnosed and confirm for first time ever. Quick celiac test «BIOHIT», yield sensitivity 95% and specificity 100%ConclusionThe prevalence of CD in children with abdominal pain was 1: 83. Quick celiac test is suitable for screening CD in population-based studies, as well as for the diagnosis of this disease in risk groups.