Pharmacological Studies on Flowers of Ceropegia bulbosa Roxb. var . bulbosa and lushii (Grah.) Hook. F from Thar Desert of Rajasthan, India

• Published in: Research journal of pharmacognosy and phytochemistry 2018-07, Vol.10 (3), p.226-232
• Main Authors: Arora, Sunita, Meena, Sonam
• Format: Article
• Language: English
• Subjects: Acids; Chemical compounds; Chromatography; Developing countries; Flowers & plants; Herbal medicine; LDCs; Mass spectrometry; Metabolites; Methods; Pharmaceutical sciences; Phytochemicals; Retention; Scientific imaging
• ISSN: 0975-2331; 0975-4385
• DOI: 10.5958/0975-4385.2018.00037.7

The phytochemical compounds were investigated using Perkin-Elmer gas chromatography-mass spectrometry, while the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology (NIST) and Willey 2008 library. In recent years Gas Chromatography- Mass Spectrometry (GC-MS) studies have been increasingly applied to analyze secondary metabolites present in the medicinal plants as this technique has been proved to be a best valuable method for the analysis of essential oil, alcohols, acids, esters, alkaloids, steroids, amino, nitro compounds etc. The solution was then boiled at 60-70° C for 18 hours on water bath, filtered, evaporated to dryness, & final residue was then subjected to GC-MS analysis that was performed at Advanced Instrumentation Research Facility (AIRF), Jawaharlal Nehru University, New Delhi, India using a GC-MS model; QP 2010 ultra-series, Shimadzu, Tokyo (Japan), equipped with thermal desorption system TD 20. Analysis of bioactive chemical compounds of Aspergillus niger by using gas chromatography-mass spectrometry and fourier-transform infrared spectroscopy.