Mango agro-industrial wastes for lipase production from Yarrowia lipolytica and the potential of the fermented solid as a biocatalyst

•Mango agroindustrial residue was tested as a substrate for the production of extracellular lipase.•Tegument was the only fraction of the residue that presented promising results.•Different nitrogen sources were used for supplementation. Yeast extract showed better results.•The highest activity valu...

Full description

Saved in:
Bibliographic Details
Published in:Food and bioproducts processing 2019-05, Vol.115, p.68-77
Main Authors: da S. Pereira, Adejanildo, Fontes-Sant’Ana, Gizele C., Amaral, Priscilla F.F.
Format: Article
Language:English
Subjects:
Agricultural wastes
agroindustrial residues
biocatalyst
Biocatalysts
Components
Enzymes
Extracellular
Fermentation
fermented solid
Industrial wastes
Inoculum
Lipase
Lipases
mango
Mangoes
Nitrogen
Nitrogen sources
Residues
Tegument
Wastes
Yarrowia lipolytica
Yeast
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Mango agroindustrial residue was tested as a substrate for the production of extracellular lipase.•Tegument was the only fraction of the residue that presented promising results.•Different nitrogen sources were used for supplementation. Yeast extract showed better results.•The highest activity value observed was 3500U/L.•A new biocatalyst was produced. Mango seed and peel, wastes from the industrial processing of the fruit, were evaluated for lipase production by Yarrowia lipolytica. Submerged fermentation of the components of mango wastes (peel, tegument and kernel) were performed with the components separately and their combinations without any supplementation. The tegument was the only part that showed promising results for lipase production and, therefore several nitrogen sources were used, separately, as supplementation. Yeast extract was the best nitrogen source for lipase production with mango tegument in submerged fermentation, achieving around 3500U/L of extracellular lipase. Optimum conditions for lipase production were pH 5.0; 187rpm of agitation speed; temperature of 27.9°C and inoculum concentration of 0.96g/L. Lipase activity of the solid residue obtained after fermentation carried out in the best experimental conditions was 68.03U/(g of residue) evidencing that the lipase produced during fermentation adsorbed in the fermented solid, producing a new biocatalyst.
ISSN:0960-3085
1744-3571
DOI:10.1016/j.fbp.2019.02.002