Peptide Ribonucleic Acid (PRNA)–Arginine Hybrids. Effects of Arginine Residues Alternatingly Introduced to PRNA Backbone on Aggregation, Cellular Uptake, and Cytotoxicity

Intracellular delivery is crucial not only in achieving effective oligonucleotide therapeutics but also for a wide variety of therapeutic strategies. In this study to elucidate the effects of the number of incorporated arginine residues on cell membrane permeability, we designed a series of peptide...

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Published in:Chemistry letters 2018-03, Vol.47 (3), p.381-384
Main Authors: Sugai, Hiroka, Nakase, Ikuhiko, Sakamoto, Seiji, Nishio, Akihiro, Inagaki, Masahito, Nishijima, Masaki, Yamayoshi, Asako, Araki, Yasuyuki, Ishibashi, Satoru, Yokota, Takanori, Inoue, Yoshihisa, Wada, Takehiko
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Language:English
Subjects:
Agglomeration
Backbone
Cell membranes
Cytotoxicity
Peptides
Residues
Ribonucleic acid
RNA
Toxicity
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cited_by cdi_FETCH-LOGICAL-c414t-d5e97db0f8988f5153e33e4b2b6c992a3da1a28ad1d87b91f3c3c4429f5de8bc3
cites cdi_FETCH-LOGICAL-c414t-d5e97db0f8988f5153e33e4b2b6c992a3da1a28ad1d87b91f3c3c4429f5de8bc3
container_end_page 384
container_issue 3
container_start_page 381
container_title Chemistry letters
container_volume 47
creator Sugai, Hiroka
Nakase, Ikuhiko
Sakamoto, Seiji
Nishio, Akihiro
Inagaki, Masahito
Nishijima, Masaki
Yamayoshi, Asako
Araki, Yasuyuki
Ishibashi, Satoru
Yokota, Takanori
Inoue, Yoshihisa
Wada, Takehiko
description Intracellular delivery is crucial not only in achieving effective oligonucleotide therapeutics but also for a wide variety of therapeutic strategies. In this study to elucidate the effects of the number of incorporated arginine residues on cell membrane permeability, we designed a series of peptide ribonucleic acids (PRNAs) that alternatingly incorporated 4, 8, or 12 arginine residues (PRR1-3) in the PRNA backbone for experimental verifications. Indeed, the cellular uptake efficiency and the aggregation properties turned out to be critical functions of the number of incorporated arginine residues. Thus, the optimized PRR2 that incorporates 8 arginines showed the most efficient cellular uptake ability, much higher than that of octaarginine, without accompanying cytotoxicity or aggregation.
doi_str_mv 10.1246/cl.171186
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source Oxford Journals Online
subjects Agglomeration
Backbone
Cell membranes
Cytotoxicity
Peptides
Residues
Ribonucleic acid
RNA
Toxicity
title Peptide Ribonucleic Acid (PRNA)–Arginine Hybrids. Effects of Arginine Residues Alternatingly Introduced to PRNA Backbone on Aggregation, Cellular Uptake, and Cytotoxicity
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