An NMR Metabolomics Approach and Detection of Ganoderma boninense-Infected Oil Palm Leaves Using MWCNT-Based Electrochemical Sensor

Ganoderma boninense (G. boninense) has been identified as a major problem in oil palm industry which caused basal stem rot disease. Identification of metabolite variation of healthy and G. boninense-infected oil palm leaves at 14 days postinfection using NMR metabolomics approach followed by charact...

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Bibliographic Details
Published in:Journal of nanomaterials 2019-01, Vol.2019 (2019), p.1-12
Main Authors: Yun, Wong Mui, Osman, Rosiah, Yusof, Nor Azah, Akanbi, Fowotade Sulayman, Isha, Azizul, Abdullah, Siti Nor Akmar
Format: Article
Language:English
Subjects:
Amino acids
Antimicrobial agents
Carbon
Chemical sensors
Chemistry
Chitosan
Chromatography
Electrochemical analysis
Electrodes
Gold
Infections
Leaves
Mass spectrometry
Metabolism
Metabolites
Multi wall carbon nanotubes
Nanomaterials
Nanoparticles
NMR
Nuclear magnetic resonance
Oxidation
Personal appearance
Physiology
Plant sciences
Principal components analysis
Scientific imaging
Sensors
Voltammetry
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Summary:Ganoderma boninense (G. boninense) has been identified as a major problem in oil palm industry which caused basal stem rot disease. Identification of metabolite variation of healthy and G. boninense-infected oil palm leaves at 14 days postinfection using NMR metabolomics approach followed by characterization of an electrochemical sensor based on a functionalized multiwalled carbon nanotube (MWCNT) layer-by-layer framework on modified screen-printed carbon electrode has been successfully determined. Significant differences from the 1H NMR data were observed between healthy and G. boninense-infected oil palm leaves, according to principal component analysis. Gold nanoparticle-functionalized MWCNT and chitosan-functionalized MWCNT were deposited on a screen-printed carbon electrode and were applied for the electrochemical detection of healthy and G. boninense-infected oil palm leaves. The electrocatalytic activities of a modified electrode towards oxidation of healthy and G. boninense-infected oil palm leaves at a concentration of 100 mg/L were evaluated using cyclic voltammetry and linear sweep voltammetry. The limits of detection of healthy and G. boninense-infected oil palm leaves were calculated to 0.0765 mg/L and 0.0414 mg/L, respectively. The modified electrode shows a good sensitivity and reproducibility due to the unique characteristics of gold nanoparticles, chitosan, MWCNTs, and synergistic interaction between them.
ISSN:1687-4110
1687-4129
DOI:10.1155/2019/4729706