Photocrosslinked acemannan-based 3D matrices for in vitro cell culture

Current biomedical research has highlighted the potential use of medicinal plant derivatives as advanced suitable biomaterials due to their biological properties. Acemannan (ACE) is the main polysaccharide of Aloe vera (AV) leaves with many reported biological properties, including immunomodulatory...

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Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2019, Vol.7 (26), p.4184-4190
Main Authors: Silva, S. S., Costa, Diana Pereira Soares, Reis, R. L.
Format: Article
Language:English
Subjects:
Acemannan
Anhydrides
Biocompatibility
Biological activity
Biological properties
Biomaterials
Biomedical applications
Biomedical materials
Biotecnologia Médica
Cell culture
Ciências Médicas
Confocal microscopy
Exploitation
Freeze drying
Gastric cancer
Herbal medicine
Immunomodulation
Irradiation
Leaves
Magnetic resonance spectroscopy
Medicinal plants
Microscopy
Mimicry
NMR
NMR spectroscopy
Nuclear magnetic resonance
Photocrosslinking
Polysaccharides
Pore size
Pore size distribution
Porosity
Scanning microscopy
Science & Technology
Size distribution
Sponges
Tumors
Ultraviolet radiation
Wound healing
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Summary:Current biomedical research has highlighted the potential use of medicinal plant derivatives as advanced suitable biomaterials due to their biological properties. Acemannan (ACE) is the main polysaccharide of Aloe vera (AV) leaves with many reported biological properties, including immunomodulatory activity, antibacterial action, wound healing induction, and cytocompatibility. These properties suggest that ACE is an appealing material for the development of 3D structures for biomedical purposes. In this study, ACE was modified by a methacrylation reaction using methacrylic anhydride. Further 3D sponges were developed by processing modified ACE by photocrosslinking using UV irradiation and the freeze-drying technique. Both FTIR and 1H NMR spectroscopy confirmed the effectiveness of the functionalization. SEM and m-CT revealed that the cross-sections of the methacrylated acemannan (MACE) sponges have open macropores and a narrow pore size distribution within them. We hypothesized that the intrinsic features of ACE could enhance tumor growth, mimicking the biological tumor microenvironment. Therefore, gastric cancer cells (AGS cell line) were cultured in contact with these matrices for up to 14 days to evaluate their cytocompatibility as a processed biomaterial. Results obtained by live-dead assay and MTS showed that cells are viable and metabolically active. Furthermore, confocal laser scanning microscopy analysis showed a homogeneous distribution of cells within the sponge. Therefore, the photocrosslinking on acemmann is proposed as an alternative approach that could significantly improve the processability of acemannan, allowing the production of high added-value structures enabling exploitation of its potential biomedical value. Portuguese Foundation for Science and Technology – FCT (SFRH/BPD/112140/2015, SFRH/BPD/85790/2012, respectively). This work was also financially supported by the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no. REGPOT-CT2012-316331-POLARIS. The authors would like to express thanks to Lorand Laboratories (Texas, USA) for the Acemannan (BiAloeÒ) supply
ISSN:2050-750X
2050-7518
DOI:10.1039/c9tb00593e