Fabrication of graphene‐silver/polyurethane nanofibrous scaffolds for cardiac tissue engineering

The graphene‐based nanocomposites are considered as great candidates for enhancing electrical and mechanical properties of nonconductive scaffolds in cardiac tissue engineering. In this study, reduced graphene oxide‐silver (rGO‐Ag) nanocomposites (1 and 2 wt%) were synthesized and incorporated into...

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Bibliographic Details
Published in:Polymers for advanced technologies 2019-08, Vol.30 (8), p.2086-2099
Main Authors: Nazari, Hojjatollah, Azadi, Shohreh, Hatamie, Shadie, Zomorrod, Mahsa Soufi, Ashtari, Khadijeh, Soleimani, Masoud, Hosseinzadeh, Simzar
Format: Article
Language:English
Subjects:
Biocompatibility
cardiac tissue engineering
Cells (biology)
Contact angle
Electric contacts
Electron microscopes
electrospinning
Graphene
Mechanical properties
Myosin
Nanocomposites
Nanofibers
Polymerase chain reaction
Polyurethane resins
Raman spectroscopy
scaffold
Scaffolds
Scanning electron microscopy
Silver
silver NPs
Tissue engineering
Transmission electron microscopy
X-ray diffraction
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Summary:The graphene‐based nanocomposites are considered as great candidates for enhancing electrical and mechanical properties of nonconductive scaffolds in cardiac tissue engineering. In this study, reduced graphene oxide‐silver (rGO‐Ag) nanocomposites (1 and 2 wt%) were synthesized and incorporated into polyurethane (PU) nanofibers via electrospinning technique. Next, the human cardiac progenitor cells (hCPCs) were seed on these scaffolds for in vitro studies. The rGO‐Ag nanocomposites were studied by X‐ray diffraction (XRD), Raman spectroscopy, and transmission electron microscope (TEM). After incorporation of rGO‐Ag into PU nanofibers, the related characterizations were carried out including scanning electron microscope (SEM), TEM, water contact angle, and mechanical properties. Furthermore, PU and PU/nanocomposites scaffolds were used for in vitro studies, wherein hCPCs showed good cytocompatibility via 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay and considerable attachment on the scaffold using SEM studies. Real‐time polymerase chain reaction (PCR) and immunostaining studies confirmed the upregulation of cardiac specific genes including GATA‐4, T‐box 18 (TBX 18), cardiac troponin T (cTnT), and alpha‐myosin heavy chain (α‐MHC) in the PU/rGO‐Ag scaffolds in comparison with neat PU ones. Therefore, these nanofibrous rGO‐Ag–reinforced PU scaffolds can be considered as suitable candidates in cardiac tissue engineering.
ISSN:1042-7147
1099-1581
DOI:10.1002/pat.4641