Cure of human diabetic neuropathy by HPLC validated bark extract of Onosma echioides L. root

HPLC validated hexane bark extract of Onosma echioides L. root (OE) was evaluated for cure of human diabetic neuropathy in human neuroblastoma cell line. HPLC analysis was performed. Human neuroblastoma cells were grouped into control, normal glucose, high glucose (HG) and HG plus different concentr...

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Bibliographic Details
Published in:Natural product research 2019-09, Vol.33 (18), p.2699-2703
Main Authors: Shoaib, Ambreen, Dixit, Rakesh Kumar, Badruddeen, Rahman, Md. Azizur, Bagga, Paramdeep, Kaleem, Sarjeel, Siddiqui, Sahabjada, Arshad, Md, Siddiqui, Hefazat Hussain
Format: Article
Language:English
Subjects:
Apoptosis
Bark
Diabetes
Diabetes mellitus
Diabetic neuropathy
Glucose
High-performance liquid chromatography
Human performance
Liquid chromatography
n-Hexane
Neuroblastoma
neuroblastoma cell
Neuroblasts
neuropathy
Onosma echioides
Oxidative stress
Plant extracts
ROS
Shikonin
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Summary:HPLC validated hexane bark extract of Onosma echioides L. root (OE) was evaluated for cure of human diabetic neuropathy in human neuroblastoma cell line. HPLC analysis was performed. Human neuroblastoma cells were grouped into control, normal glucose, high glucose (HG) and HG plus different concentrations of OE extract (10, 25 and 50 μg/mL). MTT, DCFH-DA staining and nuclear condensation assays were performed on neuroblastoma cells to evaluate antiproliferative activity, ROS activity level and apoptotic effect of OE. HPLC analysis revealed the existence of maximum yield of shikonin in n-hexane extract of OE. Exposure with different concentrations of OE effectively decreased ROS level and apoptosis of cells and as a result improved the viability of cells in a dose dependent manner in response to HG-induced oxidative stress. Thus, OE possesses the property to cure human diabetic neuropathy and further can be clinically tested for its use in diabetic neuropathy.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2018.1460838