Hollow Silica Nanoparticles as Support for Catalase Enzyme Immobilization

We have demonstrated a simple and softer fabrication technique of preparing hollow silica nanoparticles (HSNPs) immobilizing an enzyme catalase within its cavity using calcium phosphate nanoparticles as template. Morphology of the so-synthesized nanoparticles is discussed in the light of dynamic lig...

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Bibliographic Details
Published in:Catalysis letters 2013-12, Vol.143 (12), p.1304-1311
Main Authors: Singh, Henam Premananda, Gupta, Nikesh, Sharma, Rakesh Kumar
Format: Article
Language:English
Subjects:
Biological and medical sciences
Buffer solutions
Calcium phosphates
Catalase
Catalysis
Chemistry
Chemistry and Materials Science
Colloidal state and disperse state
Comparative analysis
Enzyme kinetics
Enzymes
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General and physical chemistry
Immobilization
Industrial Chemistry/Chemical Engineering
Mechanisms. Catalysis. Electron transfer. Models
Molecular biophysics
Morphology
Nanoparticles
Organometallic Chemistry
Phosphates
Photon correlation spectroscopy
Physical and chemical studies. Granulometry. Electrokinetic phenomena
Physical Chemistry
Physical chemistry in biology
Silica
Silicon dioxide
Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry
Toy industry
Transmission electron microscopy
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Summary:We have demonstrated a simple and softer fabrication technique of preparing hollow silica nanoparticles (HSNPs) immobilizing an enzyme catalase within its cavity using calcium phosphate nanoparticles as template. Morphology of the so-synthesized nanoparticles is discussed in the light of dynamic light scattering and high resolution transmission electron microscopy. The average diameter of the particles is about 50 nm. Variation of its activity over a wide range of temperature, pH and a comparison of complete enzyme kinetic study of the entrapped enzyme within the cavity of HSNPs with that of free enzyme in aqueous phosphate buffer solution are vividly discussed. Graphical Abstract
ISSN:1011-372X
1572-879X
DOI:10.1007/s10562-013-1080-9