Adsorption and activity of proteins onto mesoporous silica

The adsorption and activity of cytochrome c onto two different MCM-41 materials, MCM-41/28 and MCM-41/45 with average pore diameters of 28 and 45 Å respectively, is presented. Nitrogen gas adsorption/desorption isotherms before and after protein adsorption, and peroxidative activity profiles of the...

Full description

Saved in:
Bibliographic Details
Published in:Catalysis letters 2003, Vol.85 (1-2), p.19-23
Main Authors: DEERE, J, MAGNER, E, WALL, J. G, HODNETT, B. K
Format: Article
Language:English
Subjects:
Adsorption
Biological and medical sciences
Biotechnology
Cytochromes
Fundamental and applied biological sciences. Psychology
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
Protein adsorption
Proteins
Silicon dioxide
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The adsorption and activity of cytochrome c onto two different MCM-41 materials, MCM-41/28 and MCM-41/45 with average pore diameters of 28 and 45 Å respectively, is presented. Nitrogen gas adsorption/desorption isotherms before and after protein adsorption, and peroxidative activity profiles of the adsorbed protein demonstrate that the protein is adsorbed into the mesoporosity and remains active. The adsorption of a range of different proteins onto both MCM-41/28 and 45 shows how protein properties affect adsorption.
ISSN:1011-372X
1572-879X
DOI:10.1023/A:1022156405117