Microsatellite polymorphism and genetic differentiation in three Norwegian populations of Elymus alaskanus (Poaceae)

Variation at seven microsatellite loci was investigated in three local E. alaskanus populations from Norway and microsatellite variation was compared with allozyme variation. The percentage of polymorphic loci was 81%, the mean number of alleles per polymorphic locus was 5.7 and expected heterozygos...

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Bibliographic Details
Published in:Plant systematics and evolution 2002-11, Vol.234 (1/4), p.101-110
Main Authors: Sun, G.-L., Salomon, B., von Bothmer, R.
Format: Article
Language:English
Subjects:
Alleles
Differentiation
Gene frequency
Gene polymorphism
Genetic analysis
Genetic distance
Genetic diversity
Genetic loci
Genetic variation
Heterozygotes
Isoenzymes
Loci
Microsatellites
Natural selection
Plants
Polymerase chain reaction
Polymorphism
Population differentiation
Population genetics
Population mean
Population structure
Populations
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Summary:Variation at seven microsatellite loci was investigated in three local E. alaskanus populations from Norway and microsatellite variation was compared with allozyme variation. The percentage of polymorphic loci was 81%, the mean number of alleles per polymorphic locus was 5.7 and expected heterozygosity was 0.37. An F-statistic analysis revealed an overall 48% deficit of heterozygotes over Hardy-Weinberg expectations. Gene diversity is mainly explained by the within population component. The averaged between population differentiation coefficient, Fst, over 7 loci is only 0.13, which accounts for only 13% of the whole diversity and was contrary to allozyme analysis. The mean genetic distance between populations was 0.12. However, a χ2 -test showed that allele frequencies were different (p < 0.05) among the populations at 5 of the 7 loci. In comparison with the genetic variation detected by allozymes, microsatellite loci showed higher levels of genetic variation. Microsatellite analysis revealed that population H10576 possesses the lowest genetic variation among the tested three populations, which concur with allozyme analysis. The dendrogram generated by microsatellites agreed very well with allozymic data. Our results suggest that natural selection may be an important factor in shaping the genetic diversity in these three local E. alaskanus populations. Possible explanations for deficit heterozygosity and incongruence between microsatellites and allozymes are discussed.
ISSN:0378-2697
1615-6110
2199-6881
DOI:10.1007/s00606-002-0211-3