Genetic relationships of Iranian Hypericum perforatum L. wild populations as evaluated by ISSR markers

St. John’s wort (Hypericum perforatum L.), as the most important medicinal species of the genus Hypericum, is medicinally used for many purposes such as treatment of mild and moderate depression. Considering importance of knowledge on genetic structure of wild relatives of crop plants, the present s...

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Bibliographic Details
Published in:Plant systematics and evolution 2015-02, Vol.301 (2), p.657-665
Main Authors: Morshedloo, Mohammad Reza, Moghadam, Mohammad Reza Fattahi, Ebadi, Ali, Yazdani, Darab
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Breeding
crops
Gene flow
genes
Genetic distance
Genetic diversity
Genetic relationship
Genetic structure
Genetic variability
genetic variation
Geographical distribution
Germplasm
Hypericum perforatum
Life Sciences
Markers
microsatellite repeats
Original Article
Plant Anatomy/Development
Plant Ecology
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Population genetics
Population studies
Populations
Primers
provenance
wild relatives
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Summary:St. John’s wort (Hypericum perforatum L.), as the most important medicinal species of the genus Hypericum, is medicinally used for many purposes such as treatment of mild and moderate depression. Considering importance of knowledge on genetic structure of wild relatives of crop plants, the present study was aimed to assess genetic variability among ten wild populations of H. perforatum growing in different climatic regions of Iran via ISSR markers. In general 15 selected primers generated 191 polymorphic fragments with an average of 12 in each primer. According to the UPGMA constructed dendrogram and PCoA, studied populations were classified into four main groups which was, to the some extent, in accordance with their geographical origins. Genetic distances of these populations ranged from 0.109 (Nor and Nowshahr) to 0.345 (Ardebil and Galogah). Also, Nei gene diversity (Hₑ) values were calculated to be from 0.066 to 0.216 and Shannon’s information indices (I) varied from 0.321 to 0.097. Nei’s genetic differentiation index (Gₛₜ) was high (0.573) indicating the high levels of genetic variation among the studied populations. Finally, the gene flow (Nₘ) value was found to be 0.372. Obtained information is useful for use of Iranian H. perforatum germplasm for breeding programs and for development of effective conservation strategies.
ISSN:0378-2697
1615-6110
2199-6881
DOI:10.1007/s00606-014-1103-z