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Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)

An efficient method for producing doubled haploid plants of oilseed rape ( Brassica napus L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2012-02, Vol.108 (2), p.251-256
Main Authors: Mohammadi, Payam Pour, Moieni, Ahmad, Ebrahimi, Asa, Javidfar, Farzad
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description An efficient method for producing doubled haploid plants of oilseed rape ( Brassica napus L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for one of three treatment durations (12, 24 and 36 h) at one of the two temperatures (8 and 25°C) and were compared to control embryos (without colchicine treatment). The number of chromosomes, seed recovery, size and density of leaf stomata, and pollen grain size from regenerated plants were determined. No doubled haploid plants were regenerated from control embryos; however, the doubled haploid plants were regenerated from colchicine-treated embryos. A high doubling efficiency, 64.29 and 66.66% of regenerated plants, was obtained from 250 mg/L colchicine treatment for 24 h and 500 mg/L colchicine treatment for 36 h, respectively, at 8°C. Following 500 mg/L colchicine treatment for 36 h, a few plants regenerated (9 plants). At the higher colchicine concentration (1,000 mg/L), no plant regenerated. These results indicate that the colchicine treatment of embryos derived from microspores can induce efficient chromosome doubling for the production of doubled haploid lines of oilseed rape.
doi_str_mv 10.1007/s11240-011-0036-2
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source Springer Nature
subjects Biomedical and Life Sciences
Brassica
Chromosomes
Colchicine
Embryos
Grain size
Life Sciences
Microspores
Oilseeds
Original Paper
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Pollen
Production methods
Rape plants
Rapeseed
Stomata
title Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)
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