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Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)
An efficient method for producing doubled haploid plants of oilseed rape ( Brassica napus L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for...
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Published in: | Plant cell, tissue and organ culture tissue and organ culture, 2012-02, Vol.108 (2), p.251-256 |
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creator | Mohammadi, Payam Pour Moieni, Ahmad Ebrahimi, Asa Javidfar, Farzad |
description | An efficient method for producing doubled haploid plants of oilseed rape (
Brassica
napus
L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for one of three treatment durations (12, 24 and 36 h) at one of the two temperatures (8 and 25°C) and were compared to control embryos (without colchicine treatment). The number of chromosomes, seed recovery, size and density of leaf stomata, and pollen grain size from regenerated plants were determined. No doubled haploid plants were regenerated from control embryos; however, the doubled haploid plants were regenerated from colchicine-treated embryos. A high doubling efficiency, 64.29 and 66.66% of regenerated plants, was obtained from 250 mg/L colchicine treatment for 24 h and 500 mg/L colchicine treatment for 36 h, respectively, at 8°C. Following 500 mg/L colchicine treatment for 36 h, a few plants regenerated (9 plants). At the higher colchicine concentration (1,000 mg/L), no plant regenerated. These results indicate that the colchicine treatment of embryos derived from microspores can induce efficient chromosome doubling for the production of doubled haploid lines of oilseed rape. |
doi_str_mv | 10.1007/s11240-011-0036-2 |
format | article |
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Brassica
napus
L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for one of three treatment durations (12, 24 and 36 h) at one of the two temperatures (8 and 25°C) and were compared to control embryos (without colchicine treatment). The number of chromosomes, seed recovery, size and density of leaf stomata, and pollen grain size from regenerated plants were determined. No doubled haploid plants were regenerated from control embryos; however, the doubled haploid plants were regenerated from colchicine-treated embryos. A high doubling efficiency, 64.29 and 66.66% of regenerated plants, was obtained from 250 mg/L colchicine treatment for 24 h and 500 mg/L colchicine treatment for 36 h, respectively, at 8°C. Following 500 mg/L colchicine treatment for 36 h, a few plants regenerated (9 plants). At the higher colchicine concentration (1,000 mg/L), no plant regenerated. These results indicate that the colchicine treatment of embryos derived from microspores can induce efficient chromosome doubling for the production of doubled haploid lines of oilseed rape.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-011-0036-2</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Brassica ; Chromosomes ; Colchicine ; Embryos ; Grain size ; Life Sciences ; Microspores ; Oilseeds ; Original Paper ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Pollen ; Production methods ; Rape plants ; Rapeseed ; Stomata</subject><ispartof>Plant cell, tissue and organ culture, 2012-02, Vol.108 (2), p.251-256</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2011). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-ebe8249392220a512b2d36fa29beef80b3222305558a6fdd54f162f76bfae3a33</citedby><cites>FETCH-LOGICAL-c316t-ebe8249392220a512b2d36fa29beef80b3222305558a6fdd54f162f76bfae3a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids></links><search><creatorcontrib>Mohammadi, Payam Pour</creatorcontrib><creatorcontrib>Moieni, Ahmad</creatorcontrib><creatorcontrib>Ebrahimi, Asa</creatorcontrib><creatorcontrib>Javidfar, Farzad</creatorcontrib><title>Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>An efficient method for producing doubled haploid plants of oilseed rape (
Brassica
napus
L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for one of three treatment durations (12, 24 and 36 h) at one of the two temperatures (8 and 25°C) and were compared to control embryos (without colchicine treatment). The number of chromosomes, seed recovery, size and density of leaf stomata, and pollen grain size from regenerated plants were determined. No doubled haploid plants were regenerated from control embryos; however, the doubled haploid plants were regenerated from colchicine-treated embryos. A high doubling efficiency, 64.29 and 66.66% of regenerated plants, was obtained from 250 mg/L colchicine treatment for 24 h and 500 mg/L colchicine treatment for 36 h, respectively, at 8°C. Following 500 mg/L colchicine treatment for 36 h, a few plants regenerated (9 plants). At the higher colchicine concentration (1,000 mg/L), no plant regenerated. These results indicate that the colchicine treatment of embryos derived from microspores can induce efficient chromosome doubling for the production of doubled haploid lines of oilseed rape.</description><subject>Biomedical and Life Sciences</subject><subject>Brassica</subject><subject>Chromosomes</subject><subject>Colchicine</subject><subject>Embryos</subject><subject>Grain size</subject><subject>Life Sciences</subject><subject>Microspores</subject><subject>Oilseeds</subject><subject>Original Paper</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Pollen</subject><subject>Production methods</subject><subject>Rape plants</subject><subject>Rapeseed</subject><subject>Stomata</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp1kE9LAzEQxYMoWKsfwFvAix6ik2ST3R61_oWCFz2H7O6kRrabNdla-u1NqeDJ08C833vDPELOOVxzgPImcS4KYMA5A5CaiQMy4aqUTEFRHJIJcF0yXanymJyk9AkAWhZ8Qjb3YV132NIPO3TBt3TobD8m6kLXhY3vl7QJXfPhG98jHSPacYX9SIOjK9_EkIYQkbUY_XfOwFUdtyHt1OC7hHkV7YD08i7alHxjaW-HdaKL66tTcuRsRs5-55S8Pz68zZ_Z4vXpZX67YI3kemRYYyWKmZwJIcAqLmrRSu2smNWIroJaZkGCUqqy2rWtKhzXwpW6dhallXJKLva5Qwxfa0yj-Qzr2OeTRgg1k5UuJWSK76ndSymiM0P0Kxu3hoPZ9Wv2_Zrcr9n1a0T2iL0nZbZfYvxL_t_0A2Shfnk</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Mohammadi, Payam Pour</creator><creator>Moieni, Ahmad</creator><creator>Ebrahimi, Asa</creator><creator>Javidfar, Farzad</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20120201</creationdate><title>Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)</title><author>Mohammadi, Payam Pour ; Moieni, Ahmad ; Ebrahimi, Asa ; Javidfar, Farzad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-ebe8249392220a512b2d36fa29beef80b3222305558a6fdd54f162f76bfae3a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biomedical and Life Sciences</topic><topic>Brassica</topic><topic>Chromosomes</topic><topic>Colchicine</topic><topic>Embryos</topic><topic>Grain size</topic><topic>Life Sciences</topic><topic>Microspores</topic><topic>Oilseeds</topic><topic>Original Paper</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Pollen</topic><topic>Production methods</topic><topic>Rape plants</topic><topic>Rapeseed</topic><topic>Stomata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohammadi, Payam Pour</creatorcontrib><creatorcontrib>Moieni, Ahmad</creatorcontrib><creatorcontrib>Ebrahimi, Asa</creatorcontrib><creatorcontrib>Javidfar, Farzad</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohammadi, Payam Pour</au><au>Moieni, Ahmad</au><au>Ebrahimi, Asa</au><au>Javidfar, Farzad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2012-02-01</date><risdate>2012</risdate><volume>108</volume><issue>2</issue><spage>251</spage><epage>256</epage><pages>251-256</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>An efficient method for producing doubled haploid plants of oilseed rape (
Brassica
napus
L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for one of three treatment durations (12, 24 and 36 h) at one of the two temperatures (8 and 25°C) and were compared to control embryos (without colchicine treatment). The number of chromosomes, seed recovery, size and density of leaf stomata, and pollen grain size from regenerated plants were determined. No doubled haploid plants were regenerated from control embryos; however, the doubled haploid plants were regenerated from colchicine-treated embryos. A high doubling efficiency, 64.29 and 66.66% of regenerated plants, was obtained from 250 mg/L colchicine treatment for 24 h and 500 mg/L colchicine treatment for 36 h, respectively, at 8°C. Following 500 mg/L colchicine treatment for 36 h, a few plants regenerated (9 plants). At the higher colchicine concentration (1,000 mg/L), no plant regenerated. These results indicate that the colchicine treatment of embryos derived from microspores can induce efficient chromosome doubling for the production of doubled haploid lines of oilseed rape.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-011-0036-2</doi><tpages>6</tpages></addata></record> |
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subjects | Biomedical and Life Sciences Brassica Chromosomes Colchicine Embryos Grain size Life Sciences Microspores Oilseeds Original Paper Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Pollen Production methods Rape plants Rapeseed Stomata |
title | Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.) |
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