Somatic embryogenesis of mangosteen

Young red leaf segments from 1 to 3 year-old seedlings of mangosteen ( Garcinia mangostana L.) were incubated on different media, consisting of either B5 or Murashige and Skoog (MS) basal media and supplemented with several plant growth regulator at different levels, including 2,4-dichlorophenoxyace...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2012-08, Vol.110 (2), p.251-259
Main Authors: Rohani, Emelda Rosseleena, Ismanizan, Ismail, Noor, Normah Mohd
Format: Article
Language:English
Subjects:
2,4-D
Benzyladenine
Biomedical and Life Sciences
Dichlorophenoxyacetic acid
Embryonic growth stage
Garcinia mangostana
Growth regulators
Kinases
Kinetin
Life Sciences
Original Paper
Plant Genetics and Genomics
Plant growth
Plant Pathology
Plant Physiology
Plant Sciences
Seedlings
Somatic embryogenesis
Thidiazuron
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Summary:Young red leaf segments from 1 to 3 year-old seedlings of mangosteen ( Garcinia mangostana L.) were incubated on different media, consisting of either B5 or Murashige and Skoog (MS) basal media and supplemented with several plant growth regulator at different levels, including 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin or 2,4-D and 6-benzyladenine (BA) or thidiazuron (TDZ) and BA. The highest percentage of globular structures (36.0 %) was obtained using MS medium with a combination of TDZ and BA, each at a concentration of 0.7 mg l −1 . Histological observation of sections of globular structures showed accumulation of dense meristematic cells, a common characteristic of globular formation during somatic embryogenesis. Molecular analysis detected the gene somatic embryogenesis receptor-like kinase 1 ( SERK1 ) . This gene sequence showed a high similarity (73.0–87.0 %) to previously reported plant SERK1 .
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-012-0147-4