Regulation of hydrogen peroxide accumulation and death of Agrobacterium-transformed cells in tomato transformation

Our previous studies demonstrate that Agrobacterium causes tissue browning that subsequently reduces transformation efficiency in tomato transformation. In addition application of lipoic acid (LA) can reduce tissue browning and increase transformation efficiency in different crops. A major challenge...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2016-10, Vol.127 (1), p.229-236
Main Authors: Dan, Yinghui, Zhang, Song, Matherly, Amanda
Format: Article
Language:English
Subjects:
Accumulation
Agrobacterium
Biomedical and Life Sciences
Browning
Cell death
Correlation analysis
Gene expression
Gene regulation
Genetic transformation
Hydrogen peroxide
Life Sciences
Lipoic acid
Molecular modelling
Original Article
Oxidative stress
Peroxiredoxin
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant production
Plant Sciences
Reactive oxygen species
Scavenging
Superoxide dismutase
Tomatoes
Transformed cells
Transgenic plants
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Summary:Our previous studies demonstrate that Agrobacterium causes tissue browning that subsequently reduces transformation efficiency in tomato transformation. In addition application of lipoic acid (LA) can reduce tissue browning and increase transformation efficiency in different crops. A major challenge in Agrobacterium -mediated plant transformation is the death of Agrobacterium -transformed cells (DATC), which restricts transgenic plant production. Hydrogen peroxide (H 2 O 2 ) plays a critical role in oxidative stress. However, little is known about the biochemical and molecular mechanisms for DATC. Our biological and correlation analyses showed that Agrobacterium mediated H 2 O 2 accumulation (HOA) and HOA elevation led to DATC during Agrobacterium -mediated tomato cv. MicroTom transformation. Agrobacterium significantly ( P  
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-016-1045-y