Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

The aim of this paper was to develop a protocol for efficient storage of artificial seeds of Taraxacum pieninicum , critically endangered Asteraceae species. Storage under reduced light conditions or in the darkness was tested on a basis of synthetic seeds ability to conversion and post-storage regr...

Full description

Saved in:
Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2018-03, Vol.132 (3), p.469-478
Main Authors: Kamińska, Monika, Gołębiewski, Marcin, Tretyn, Andrzej, Trejgell, Alina
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cytometry
Darkness
Endangered species
Flow cytometry
Genetic diversity
Growth conditions
Life Sciences
Necrosis
Original Article
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Regrowth
Seeds
Shoots
Subculture
Subcultures
Taraxacum
Tips
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this paper was to develop a protocol for efficient storage of artificial seeds of Taraxacum pieninicum , critically endangered Asteraceae species. Storage under reduced light conditions or in the darkness was tested on a basis of synthetic seeds ability to conversion and post-storage regrowth of shoot tips. The results indicated that synseeds obtained from shoot tips of T. pieninicum can be stored at 4 °C even for 12 months without subculture. The light is a stress factor during storage what was manifested by numerous necrosis and decreased shoots ability to proliferate in optimal growth conditions in 1st subculture. Additionally our results showed that the storage does not produce genetic variation at the resolution provided by the flow cytometry and RAPD analysis.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-017-1343-z