High performance separation technologies and spectroscopic tools for plant extract characterization in phytomics

The review is concerned with fast analytical methods for the qualitative and quantitative determination of plant constituents and phytopharmaceutical products. Emphasis is put on the determination of leading compounds, the role of the stationary phase in the analysis of sugars, phenolic compounds, p...

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Bibliographic Details
Published in:Phytochemistry reviews 2002-10, Vol.1 (3), p.413-426
Main Authors: Stecher, G., Huck, C.W., Stöggl, W.M., Guggenbichler, W., Bakry, R., Bonn, G.K.
Format: Article
Language:English
Subjects:
Chromatography
High performance liquid chromatography
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Mutation
Near infrared radiation
Phenolic compounds
Phenols
Plant extracts
Qualitative analysis
Reflectance
Stationary phase
Sugar
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Summary:The review is concerned with fast analytical methods for the qualitative and quantitative determination of plant constituents and phytopharmaceutical products. Emphasis is put on the determination of leading compounds, the role of the stationary phase in the analysis of sugars, phenolic compounds, proteins or DNA-mutations and the use of non-standard analytical tools such as micro high performance liquid chromatography (μ-LC) or near infrared reflectance spectroscopy (NIR) next to conventional high performance liquid chromatography (HPLC). Finally the importance of spectroscopic methods such as mass spectrometry (MS) in phytomics will be discussed giving examples for HPLC-MS and μ-HPLC-MS hyphenation.
ISSN:1568-7767
1572-980X
DOI:10.1023/A:1026083122460