Identification of promoter exchange at a male fertility restorer locus for cytoplasmic male sterility in radish (Raphanus sativus L.)

The cytoplasmic male sterility (CMS)/nucleus-controlled fertility restoration (Rf) system is a major component that exploits hybrid vigor in crops. Here, we have identified a new pentatricopeptide repeat (PPR)-encoding allele at the RsRf3 locus linked to the fertility restoration to CMS in radish. T...

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Bibliographic Details
Published in:Molecular breeding 2017-06, Vol.37 (6), p.1-6, Article 82
Main Authors: Wang, Zhi Wei, De Wang, Chuan, Cai, Qing Ze, Mei, Shi Yong, Gao, Lei, Zhou, Yuan, Wang, Ting
Format: Article
Language:English
Subjects:
Alleles
Biomedical and Life Sciences
Biotechnology
Crossovers
Cytoplasmic male sterility
Fertility
Gene expression
Hybrid vigor
Life Sciences
Loci
Male sterility
Mitochondria
Molecular biology
Nuclei
Plant biology
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Proteins
Radishes
Raphanus sativus
Restoration
Ribonucleic acid
RNA
Short Communication
Vigor
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Summary:The cytoplasmic male sterility (CMS)/nucleus-controlled fertility restoration (Rf) system is a major component that exploits hybrid vigor in crops. Here, we have identified a new pentatricopeptide repeat (PPR)-encoding allele at the RsRf3 locus linked to the fertility restoration to CMS in radish. This allele was named as RsRf3-5 and encodes a putative protein with 18 PPRs and a predicted mitochondrial targeting signal. Another allele RsRf3-6 encoding the same protein with RsRf3-5 possesses a different promoter region and has failed to restore fertility to CMS. The non-restoring allele RsRf3-7 encoding a predicted protein with 15 PPRs has an identical promoter region with RsRf3-6 , suggesting that RsRf3-6 could result from a recent crossover between RsRf3-5 and RsRf3-7 . Interestingly, the RsRf3-5 allele shows higher RNA expression levels in the CMS cytoplasmic background compared with the RsRf3-6 allele.
ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-017-0683-z