Mapping QTLs for submergence tolerance in rice using a population fixed for SUB1A tolerant allele

• Published in: Molecular breeding 2017-04, Vol.37 (4), p.1-10, Article 47
• Main Authors: Gonzaga, Zennia Jean C., Carandang, Jerome, Singh, Anshuman, Collard, Bertrand C.Y., Thomson, Michael J., Septiningsih, Endang M.
• Format: Article
• Language: English
• Subjects: Alleles; Biomedical and Life Sciences; Biotechnology; Chromosome 2; Chromosome 8; Chromosome 9; Chromosomes; Crop production; Cultivars; Flooding; Gene loci; Gene mapping; Genetics; Genotyping; Inbreeding; Life Sciences; Mapping; Marker-assisted selection; Molecular biology; Oryza; Phenotypic variations; Phenotyping; Plant biology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Population genetics; Quantitative trait loci; Single-nucleotide polymorphism; Sub1 gene; Submergence; Variance
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-017-0637-5

Submergence is a widespread problem of rice production, especially in low-lying areas in South and Southeast Asia. Despite the success of Sub1 mega varieties, repeated instances of prolonged and severe flooding in stress-prone areas suggests that the SUB1 gene is no longer sufficient in those regions and requires improved varieties with increased tolerance. A study was conducted to identify quantitative trait loci (QTLs) associated with submergence tolerance using 115 F 7 recombinant inbred lines (RILs) derived from the cross of Ciherang-Sub1, a popular Indonesian cultivar carrying the SUB1 gene that has relatively higher tolerance to submergence compared to the performance of most other Sub1 lines and the submergence and stagnant flooding tolerant IR10F365. As the tolerant allele at SUB1A on chromosome 9 was fixed in this mapping population, additional QTLs responsible for submergence tolerance were expected to be revealed. Genotyping with an Infinium 6K SNP chip resulted in 469 polymorphic markers that were then used for QTL mapping. Phenotyping was performed under complete submergence with two replicates. A major QTL for submergence derived from Ciherang-Sub1, named qSUB8.1 , was detected on chromosome 8 with a LOD score of 10.3 and phenotypic variance of 27.5%. Additionally, a smaller QTL, also derived from Ciherang-Sub1, was detected on chromosome 2 with a LOD score of 3.5 and phenotypic variance of 12.7%. There was no digenic interaction detected between these QTLs suggesting their independent action. The QTLs detected in this study can be used in marker-assisted selection to further improve the tolerance of other Sub1 varieties.