Development and validation of donor-specific STS markers for tracking alien introgressions into Brassica juncea (L.) Czern

Alien introgressions into crop plants rely on phenotypic evaluation. Employing molecular markers could greatly accelerate this and help discover new alleles/QTLs. We report here a new strategy to develop markers for tracking introgression using genome survey sequence. We demonstrate this using an ad...

Full description

Saved in:
Bibliographic Details
Published in:Molecular breeding 2017-09, Vol.37 (9), p.1-10, Article 110
Main Authors: Vasupalli, Naresh, Rao, Mahesh, Chamola, Rohit, Pant, Usha, Bhattacharya, Ramcharan, Bhat, Shripad Ramachandra
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biotechnology
Brass plating
Brassica
Brassica juncea
Diplotaxis erucoides
Genetics
Genomes
Genotyping
Life Sciences
Markers
Molecular biology
Nucleotide sequence
Phenotypes
Plant biology
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Polymorphism
Quantitative trait loci
Tracking
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Alien introgressions into crop plants rely on phenotypic evaluation. Employing molecular markers could greatly accelerate this and help discover new alleles/QTLs. We report here a new strategy to develop markers for tracking introgression using genome survey sequence. We demonstrate this using an advanced backcross population of Brassica juncea involving the wild species Diplotaxis erucoides . To develop D. erucoides -specific markers, 72 million single end reads were obtained using Ion-Torrent platform. Quality reads (67.6 million) were checked against Brassica database and the redundant reads were eliminated. De novo assembly of the remaining 14.6 million reads gave 3895 contigs (> 1 kb), which were used to design 101 donor-specific (DS) STS markers. Of these, 89 markers showed polymorphism between D. erucoides and B. juncea . Genotyping of 90 randomly picked plants with 31 donor-specific STS markers detected 22 plants containing 17 markers. Alien introgression was also detected in eight of the 22 lines displaying phenotypes deviating from B. juncea parent. The marker DSSTS 70 was found in six of the nine lines showing glossy leaf suggesting its association with the trait. This is the first study demonstrating the use of molecular markers for implementing reverse genetics approach for alien introgression into crop plants.
ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-017-0714-9