Marker-assisted introgression of bacterial blight and blast resistance into DRR17B, an elite, fine-grain type maintainer line of rice
DRR17A is a stable wild-abortive cytoplasmic male sterile line with medium-slender grain type. DRR17A and its maintainer line DRR17B are highly susceptible to two of the major rice diseases, bacterial blight (BB) and blast. To improve DRR17B for resistance against BB and blast, we have introgressed...
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Published in: | Molecular breeding 2015-07, Vol.35 (7), p.1-12, Article 151 |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
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Online Access: | Get full text |
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Summary: | DRR17A is a stable wild-abortive cytoplasmic male sterile line with medium-slender grain type. DRR17A and its maintainer line DRR17B are highly susceptible to two of the major rice diseases, bacterial blight (BB) and blast. To improve DRR17B for resistance against BB and blast, we have introgressed a major dominant gene each conferring resistance against BB (
Xa21
) and blast (
Pi54
) into the maintainer line through marker-assisted backcross breeding using RP-Bio-Patho-2 (a near-isogenic line of Samba Mahsuri possessing
Xa21
and
Pi54
) as the donor parent. PCR-based molecular markers tightly linked to
Xa21
and
Pi54
were used for foreground selection of the resistance plants at each backcross generation, while molecular markers tightly linked to the major fertility restorer genes,
Rf3
and
Rf4
, were used for negative selection (i.e. selection of plants possessing non-fertility-restoring alleles at the two loci) at BC
1
generation. After foreground selection for the target genes at each backcross generation, the ‘positive’ plants were screened with parental polymorphic markers for identifying backcross plants possessing maximum recovery of DRR17B genome. Marker-assisted backcrossing was continued till BC
3
generation, and a single BC
3
F
1
plant possessing the target genes with ~94 % recovery of recurrent parent genome was identified and selfed to generate BC
3
F
2
s. A total of six homozygous BC
3
F
2
plants were identified and advanced. At BC
3
F
5
, six promising, stable, backcross-derived lines possessing high level of resistance against BB and blast, high yield, short plant stature, fine-grain type, have been identified; their maintenance ability and heterotic potential validated through test crosses and these lines are being converted to CMS lines through marker-assisted breeding. |
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ISSN: | 1380-3743 1572-9788 |
DOI: | 10.1007/s11032-015-0348-8 |