Angiogenic potential of boron-containing bioactive glasses: in vitro study

Boron-containing bioactive glasses (BGs) are being extensively researched for the treatment and regeneration of bone defects because of their osteostimulatory and neovascularization potential. In this study, we report the effects of the ionic dissolution products (IDPs) of different boron-doped, bor...

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Bibliographic Details
Published in:Journal of materials science 2017-08, Vol.52 (15), p.8785-8792
Main Authors: Balasubramanian, P., Hupa, L., Jokic, B., Detsch, R., Grünewald, A., Boccaccini, Aldo R.
Format: Article
Language:English
Subjects:
Biocompatibility
Bioglass
Biological activity
Biomedical materials
Bone marrow
Boron
Boron oxides
Characterization and Evaluation of Materials
Chemistry and Materials Science
Classical Mechanics
Crystallography and Scattering Methods
Enzyme-linked immunosorbent assay
Growth factors
In Honor of Larry Hench
Materials Science
Morphology
Neovascularization
Phosphorus pentoxide
Polymer Sciences
Regeneration
Scaffolds
Silicon dioxide
Solid Mechanics
Vascular endothelial growth factor
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Summary:Boron-containing bioactive glasses (BGs) are being extensively researched for the treatment and regeneration of bone defects because of their osteostimulatory and neovascularization potential. In this study, we report the effects of the ionic dissolution products (IDPs) of different boron-doped, borosilicate, and borate BG scaffolds on mouse bone marrow stromal cells in vitro, using an angiogenesis assay. Five different BG scaffolds of the system SiO 2 –Na 2 O–K 2 O–MgO–CaO–P 2 O 5 –B 2 O 3 (with varying amounts of SiO 2 and B 2 O 3 ) were fabricated by the foam replication technique. Bone marrow stromal cells were cultivated in contact with the IDPs of the boron-containing BG scaffolds at different concentrations for 48 h. The expression and secretion of vascular endothelial growth factor (VEGF) from the cultured cells was measured quantitatively using the VEGF ELISA Kit. Cell viability and cell morphology were determined using WST-8 assay and H&E staining, respectively. The cellular response was found to be dependent on boron content and the B release profile from the glasses corresponded to the positive or negative biological activity of the BGs.
ISSN:0022-2461
1573-4803
DOI:10.1007/s10853-016-0563-7