Use of autoclave extraction and liquid chromatography with tandem mass spectrometry for determination of maleic hydrazide residues in tobacco

Maleic hydrazide has been extensively used as an effective growth regulator in tobacco sucker control. After application, maleic hydrazide distributes itself throughout the tobacco plant where it can exist as free, or forms glucoside conjugates with glucose, or becomes bound with lignin. Among them,...

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Bibliographic Details
Published in:Journal of separation science 2019-07, Vol.42 (14), p.2390-2397
Main Authors: Deng, Huimin, Bian, Zhaoyang, Yang, Fei, Liu, Shanshan, Li, Zhonghao, Fan, Ziyan, Wang, Ying, Tang, Gangling
Format: Article
Language:English
Subjects:
Acidic water
autoclave extraction
Autoclaving
Chromatography
Chromatography, Liquid
Conjugates
Growth regulators
Hydrochloric acid
Lignin
Linearity
Liquid chromatography
maleic hydrazide
Maleic Hydrazide - analysis
Mass spectrometry
Nicotiana - chemistry
Pesticide Residues - analysis
Residues
Scientific imaging
Solvent extraction
Spectroscopy
Tandem Mass Spectrometry
Tobacco
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Summary:Maleic hydrazide has been extensively used as an effective growth regulator in tobacco sucker control. After application, maleic hydrazide distributes itself throughout the tobacco plant where it can exist as free, or forms glucoside conjugates with glucose, or becomes bound with lignin. Among them, free maleic hydrazide and its glucoside conjugates are extractable under conventional solvent extraction, while lignin bound maleic hydrazide is claimed to be non‐extractable. Herein, an autoclave extraction method has been developed to extract maleic hydrazide effectively, in which tobacco samples are extracted in an autoclave at 130°C for 1 h using 4 M hydrochloric acid. Under such pressurized hot acidic water conditions, lignin bound maleic hydrazide can be released. Meanwhile, glucoside conjugates are hydrolyzed. Total maleic hydrazide is detected by liquid chromatography coupled with tandem mass spectrometry, and the quantitative results coincide well with that obtained from the international standard method. The proposed autoclave extraction with liquid chromatography and tandem mass spectrometry method exhibits excellent linearity in the range of 5–200 mg/kg (R2 = 0.9998), the matrix matched limit of detection and limit of quantification is 0.68 and 2.27 mg/kg, respectively. This method is simple and improves sample capacity, providing an effective approach to monitoring maleic hydrazide residues in tobacco.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201900250