Phylogenetic relationship of 40 species of genus Aloe L. and the origin of an allodiploid species revealed by nucleotide sequence variation in chloroplast intergenic space and cytogenetic in situ hybridization

Aloe species, which have been used as medicinal plants, belong to the Asphodelaceae family consisting of 530 species. In this study, genetic diversity and phylogenetic relationships among 40 Aloe species including a putative interspecies hybrid were analyzed using PCR band profiles from eight chloro...

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Bibliographic Details
Published in:Genetic resources and crop evolution 2016-02, Vol.63 (2), p.235-242
Main Authors: Lee, Yun Sun, Park, Hye Mi, Kim, Nam-Hoon, Waminal, Nomar E., Kim, Yeon Jeong, Lim, Ki-Byung, Baek, Jin Hong, Kim, Hyun Hee, Yang, Tae-Jin
Format: Article
Language:English
Subjects:
Aloe
Biodiversity
Chloroplasts
Chromosomes
Cytogenetics
Fluorescence
Gene sequencing
Genera
Genetic diversity
Genomes
Herbal medicine
Hybridization
Karyotypes
Medicinal plants
Nucleotide sequence
Nucleotides
Phylogenetics
Phylogeny
Species
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Summary:Aloe species, which have been used as medicinal plants, belong to the Asphodelaceae family consisting of 530 species. In this study, genetic diversity and phylogenetic relationships among 40 Aloe species including a putative interspecies hybrid were analyzed using PCR band profiles from eight chloroplast intergenic space markers and nucleotide sequence diversity in the psbK–psbI intergenic region. A phylogenetic tree based on psbK–psbI sequences supported the revised classification of the genus Aloe as polyphyletic with several species be re-allocated into three genera Kumara, Aloidendron, and Aloiampelos. Further, the origin of the putative interspecies Aloe hybrid was characterized through molecular cytogenetics. Fluorescence and genomic insitu hybridization illustrated that the hybrid has a bimodal karyotype with a chromosome complement of 2n = 14, of which complementary halves were derived from two parental species, A. vera and A. arborescens. These findings revealed that the hybrid species was allodiploid. The phylogenetic analysis showed that A. arborescens was the maternal genome donor of the hybrid, as both have identical chloroplast genome sequences. We thus conclude that the allodiploid hybrid should be called A. arborescens × A.vera.
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-015-0243-5